Optimization of Purification Technology for Tea Polyphenol from Tea Stalk by Macroporous Resin

CHEN Pei-xin; GAO Ying; LI Wei-min; LI Jing

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Optimization of Purification Technology for Tea Polyphenol from Tea Stalk by Macroporous Resin

更新时间：2024-01-04

- Optimization of Purification Technology for Tea Polyphenol from Tea Stalk by Macroporous Resin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 22, Pages: 65-68(2012)
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- Published：2012
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CHEN Pei-xin, GAO Ying, LI Wei-min, et al. Optimization of Purification Technology for Tea Polyphenol from Tea Stalk by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 65-68.
DOI：

CHEN Pei-xin, GAO Ying, LI Wei-min, et al. Optimization of Purification Technology for Tea Polyphenol from Tea Stalk by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(22): 65-68. DOI：

摘要

目的: 优选大孔吸附树脂纯化茶梗中茶多酚的工艺条件

为茶梗二次开发提供参考。方法: 采用静态吸附-解吸和动态吸附-解吸试验对9种不同型号树脂进行筛选;以茶多酚含量为指标

采用单因素试验优选大孔树脂纯化茶多酚的工艺参数。结果: LX-18型大孔吸附树脂纯化效果最好;最佳工艺条件为上样液中生药与树脂的质量比1∶1

吸附速度2 mL·min-1

分别用10倍量水、50%乙醇洗脱;茶多酚纯度53.79%。结论: 该优选的方法简便可行、纯化效果好

可为茶多酚相关产品的开发提供参考。

Abstract

Objective:To optimize purification technology of tea-polyphenol from tea stalk by macroporous resin

and provide a reference for secondary development of tea stalk. Method: Static absorption-desorption and dynamic adsorption-desorption was used to select the best one from 9 different type macroporous resin;With the content of tea-polyphenol as index

purification technology parameters of tea polyphenol were optimized by single factor test. Result: LX-18 type resin showed the best purifying profile

its optimum technology conditions were as follows:crude drugs-dry resin weight 1∶1

adsorption rate of 2 mL·min-1

eluted by 10 BV distilled water and 10 BV 50% ethanol;Purity of tea polyphenol was 53.79%. Conclusion: This optimized technology was simple and feasible with good purification effect

so it could provide a reference for development of related products of tea polyphenol.

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