Protective Effects and Molecular Mechanism of Ligustrazine and Ferulate on Myocardial Ischemia-reperfusion Injury in Rats

ZHAO Run-ying; HAO Wei; MENG Xiang-jun; ZHAO Li-ni; LI Zhao; WANG Jun-ping; DING Shuang-shuang; WEI Wei

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Protective Effects and Molecular Mechanism of Ligustrazine and Ferulate on Myocardial Ischemia-reperfusion Injury in Rats

更新时间：2024-01-04

- Protective Effects and Molecular Mechanism of Ligustrazine and Ferulate on Myocardial Ischemia-reperfusion Injury in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 19, Pages: 230-234(2012)
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- Published：2012
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ZHAO Run-ying, HAO Wei, MENG Xiang-jun, et al. Protective Effects and Molecular Mechanism of Ligustrazine and Ferulate on Myocardial Ischemia-reperfusion Injury in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 230-234.
DOI：

ZHAO Run-ying, HAO Wei, MENG Xiang-jun, et al. Protective Effects and Molecular Mechanism of Ligustrazine and Ferulate on Myocardial Ischemia-reperfusion Injury in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 230-234. DOI：

摘要

目的:观察阿魏酸川芎嗪对大鼠心肌缺血再灌注损伤的影响

并探讨其可能的分子机制。方法:将60只雄性SD大鼠随机分成5组:假手术组、缺血再灌注组、川芎嗪(4mg·kg-1)组、阿魏酸川芎嗪低剂量(4mg·kg-1)组、阿魏酸川芎嗪高剂量(8mg·kg-1)组;采用结扎左冠状动脉前降支30min、再灌注120min的方法复制大鼠心肌缺血再灌注模型;各组大鼠于再灌注前10min分别颈iv给药;于再灌注结束后

进行血清生化学指标及心肌组织学检测。结果:阿魏酸川芎嗪能显著降低血清中肌酸激酶同功酶(CK-MB)、乳酸脱氢酶(LDH)和心肌钙蛋白I(cTnI)、丙二醛(MDA)的水平

提高总超氧化物歧化酶(T-SOD)活性

缩小心肌梗死范围

增加Bcl-2蛋白的表达

减少Bax蛋白的表达

降低Bcl-2/Bax的比值和心肌细胞凋亡指数

与缺血再灌注组比较

差异有统计学意义(P＜0.01);阿魏酸川芎嗪部分指标优于川芎嗪(P＜0.05)

且呈现剂量依赖性。结论:阿魏酸川芎嗪对大鼠心肌缺血再灌注损伤具有良好保护作用;阿魏酸川芎嗪抗心肌细胞凋亡的机制可能与其上调Bcl-2基因和下调Bax基因表达有关。

Abstract

Objective:To observe the protective effect of ligustrazine and ferulate on myocardial ischemia-reperfusion injury and probe into its possible molecular mechanism in rats. Method:Sixty male rats were randomly divided into five groups:sham-operation group

ischemia-reperfusion group

ligustrazine(4 mg·kg-1) group

ligustrazine+ferulate low dose(4 mg·kg-1) group

ligustrazine+ferulate high dose(8 mg·kg-1) group.The rat model with ischemia-reperfusion injury was established with 30 min of myocardial ischemia and followed by 120 min reperfusion.The rats were treated for 10 min before reperfusion through jugular vein injection.After the reperfusion was finished

the biochemical indicators in serum and histological index in myocardium were investigated. Result:Compared with ischemia-reperfusion group

ligustrazine and ferulate could lower the level of creatine kinase isoenzyme(CK-MB)

lactate dehydrogenas(LDH)

cardiac troponin I(cTnI) and malondialdehyde(MDA) in blood

increase the activity of total-superoside dismutase(T-SOD) in blood

reduce the myocardial infarction size

increase the expression of Bcl-2 protein

decrease the expression of Bax protein

reduce the myocardial apoptosis index and Bcl-2/Bax ratio(P＜0.01);all indicators for ligustrazine and ferulate showed dose-dependently superior to ligustrazine(P＜0.05或P＜0.01). Conclusion:Ligustrazine and ferulate had a well protective effect on rats with myocardial ischemia-reperfusion injury.Anti-apoptotic effect was related to up-regulate the expression of Bcl-2 and down-regulate the expression of Bax by ligustrazine and ferulate.

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Related Institution

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