Effects of Extract of on Melanin Synthesis in Co-culture System of Human Melanoma and Keratinocytes

CHEN Jing-hua; WANG Xing-yan; WANG Xue; CHEN Qiao-yun; ZHANG Ning

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Effects of Extract of on Melanin Synthesis in Co-culture System of Human Melanoma and Keratinocytes

更新时间：2024-01-04

- Effects of Extract of on Melanin Synthesis in Co-culture System of Human Melanoma and Keratinocytes
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 17, Pages: 205-208(2012)
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- Published：2012
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CHEN Jing-hua, WANG Xing-yan, WANG Xue, et al. Effects of Extract of on Melanin Synthesis in Co-culture System of Human Melanoma and Keratinocytes[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(17): 205-208.
DOI：

CHEN Jing-hua, WANG Xing-yan, WANG Xue, et al. Effects of Extract of on Melanin Synthesis in Co-culture System of Human Melanoma and Keratinocytes[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(17): 205-208. DOI：

摘要

目的: 考察当归水提取物对体外构建的人黑素瘤细胞(A375)与角质形成细胞(HaCaT)共培养模型黑素合成的影响。方法: 分别培养A375细胞与HaCaT细胞

A375细胞与HaCaT细胞以1∶2的比例接种于培养板后加入MEM培养基培养24 h。用MEM将当归提取物制备成0.1~2 g·L-1 5个质量浓度

细胞给药作用48 h

采用MTT 法、NaOH 裂解法、多巴氧化法分别测定给各组 A375 细胞与 HaCaT 细胞共培养模型的细胞活力、黑素含量和酪氨酸酶活性。结果: 与阴性对照组相比

0.5 g·L-1当归提取物对共培养细胞的增殖有显著促进作用(P＜0.01);2

0.5

0.25 g·L-1当归提取物对黑素合成有显著的抑制作用(P＜0.05或P＜0.01)

呈现浓度依赖性的抑制作用趋势;当归提取物各浓度组对酪氨酸酶活性均有显著抑制作用

1 g·L-1组的抑制作用弱于熊果苷组(P＜0.01)

0.5

0.25

0.125 g·L-1组与熊果苷组作用相当。结论: 当归水提取物能够通过抑制酪氨酸酶活性来抑制 A375 细胞和 HaCaT 细胞共培养体系的黑素合成

为临床使用当归治疗黄褐斑提供了实验依据。

Abstract

Objective: To study the effects of the extract of Angelica sinensis on the melanin synthesis in co-culture system of human melanoma and keratinocytes cells. Method: A375 and HaCaT cell were cultured respectively in MEM and co-cultured in 1∶2 ratio for 24 hours. The co-cultured cells were cultured for 48 hours after MEM containing different concentration of A. sinensis were added to the dishes. The proliferation

the tyrosinase(TYR) activity and melanin contents of the co-culture cells were measured by MTT

dopa-oxidase and NaON assays respectively. Result: Compared with control group

the cells proliferation were markedly increased by the extract of A. sinensis in the concentration range of 0.5-2 g·L-1(P＜0.01)

the melanin synthesis were markedly suppressed in the concentration range of 0.25-2 g·L-1(P＜0.05 or P＜0.01)

TYR activity were significantly suppressed in the concentration range of 0.125-2 g·L-1(P＜0.01). Conclusion: The water extract of A. sinensis can decrease the melanogenesis by decreasing the activity of tyrosinase though it can increase the proliferation of the cells in higher concentration.

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