Antioxidant Activity of Constituents from

DANG Cui-zhi; HANG Xiao-yan; YANG Qing-xiong; AI Jun-li; TIAN Meng

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Antioxidant Activity of Constituents from

更新时间：2024-01-04

- Antioxidant Activity of Constituents from
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 17, Pages: 100-103(2012)
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- Published：2012
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DANG Cui-zhi, HANG Xiao-yan, YANG Qing-xiong, et al. Antioxidant Activity of Constituents from[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(17): 100-103.
DOI：

DANG Cui-zhi, HANG Xiao-yan, YANG Qing-xiong, et al. Antioxidant Activity of Constituents from[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(17): 100-103. DOI：

摘要

目的: 研究灯盏细辛6个化合物的抗氧化活性。方法: 以灯盏细辛为原料

采用有机溶剂提取法和色谱柱法对灯盏细辛中化学成分进行提取与分离

利用核磁共振等波谱学方法进行结构鉴定

体外法检测6个化合物对1-二苯基-2-苦味酰肼自由基清除法(DPPH)

2'-联氨-双-(3-乙基苯并噻唑啉-6-磺酸)自由基清除法(ABTS)

O2-·自由基和·OH自由基的清除能力

并以维生素C(Vc)为阳性对照

结果: 6个化合物者对DPPH

ABTS

O2-·

·OH自由基均具有清除作用

且与浓度呈良好的量效关系

6个化合物清除自由基能力与Vc相似。结论: 灯盏细辛中的6个化合物均具有较强的抗氧化活性。

Abstract

Objective: To study the antioxidant activity of 6 compounds isolated from Erigeron breviscapus. Method: Compounds were isolated and acquired from E. breviscapus through the methods of organic solvent extraction and chromatography

and identified by the spectrum of NMR(nuclear magnetic resonance). The scavenging activities of all these compounds against 1

1-diphenyl-2-picrylhydrazyl radical (DPPH) radicals

2'-amino-di(2-ethyl-benzothiazoline sulphonic acid-6) ammonium salt (ABTS) radicals

O2-·and·OH radicals were evaluated by in vitro method and compared with Vc as positive control. Result: All the six compounds have scavenging capability against DPPH radicals

ABTS radicals

O2-·and·OH radicals and have a good dose-responses relationship with the concentration. The differences between the free radical scavenging capacity of six compounds and Vc are were significant. Conclusion: Six pounds isolated from E. breviscapus have the stronger antioxidant activity.

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Related Institution

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Henan Provincial Engineering Technology Research Center for Quality Control and Evaluation of Traditional Chinese Medicine， Henan University of Chinese Medicine

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