Study on HPLC-ELSD Fingerprints of Bulbus Fritillariae Pallidiflorae and the Determination of Sipeimine in Them
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Study on HPLC-ELSD Fingerprints of Bulbus Fritillariae Pallidiflorae and the Determination of Sipeimine in Them
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 21, Pages: 82-85(2012)
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Published:2012
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CAI Xiao-cui, HE Jin-hua, XU Fang, et al. Study on HPLC-ELSD Fingerprints of Bulbus Fritillariae Pallidiflorae and the Determination of Sipeimine in Them[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(21): 82-85.
DOI:
CAI Xiao-cui, HE Jin-hua, XU Fang, et al. Study on HPLC-ELSD Fingerprints of Bulbus Fritillariae Pallidiflorae and the Determination of Sipeimine in Them[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(21): 82-85.DOI:
Study on HPLC-ELSD Fingerprints of Bulbus Fritillariae Pallidiflorae and the Determination of Sipeimine in Them
Objective: To establish HPLC-ELSD fingerprints of Bulbus Fritillariae Pallidiflorae and determine the content of sipeimine in them. Method: The HPLC-ELSD fingerprints of Bulbus Fritillariae Pallidiflorae from different places were obtained. The HPLC separation was performed on a Cosmosil Cl8 column (4.6 mm×250 mm
5 μm) in gradient elute mode with a mixture consisting of acetonitrile and 0.03% diethylamine solution at a flow rate of 1.0 mL·min-1.The ELSD detector is used and the drift tube temperature of ELSD was set at 67℃ with the flow rate of 2.0 L·min-1. Result: The HPLC-ELSD fingerprints were set up and the similar degrees to the crude drugs collected in different places were compared. The content of sipeimine was determined. Conclusion: The method is simple and reproducible and can be used for the quality control of Bulbus Fritillariae Pallidiflorae.