Ultra-Performance Liquid Chromatography for Quantification of Flavone in  Preparation

HAN Liang; YU Chu-qin; LIN Hua-qing

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Ultra-Performance Liquid Chromatography for Quantification of Flavone in Preparation

更新时间：2024-01-04

- Ultra-Performance Liquid Chromatography for Quantification of Flavone in Preparation
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 13, Pages: 115-118(2012)
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- Published：2012
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HAN Liang, YU Chu-qin, LIN Hua-qing. Ultra-Performance Liquid Chromatography for Quantification of Flavone in Preparation[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 115-118.
DOI：

HAN Liang, YU Chu-qin, LIN Hua-qing. Ultra-Performance Liquid Chromatography for Quantification of Flavone in Preparation[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 115-118. DOI：

摘要

目的: 采用UPLC-DAD技术快速测定黄芪及其制剂中主要黄酮类成分的含量。方法: 采用超高效液相色谱仪

用ACQUITY UPLC TMBEH C18柱(2.1 mm×50 mm

1.7 μm);流动相由甲醇-0.2%甲酸水溶液线性梯度洗脱;流速0.5 mL·min-1

检测波长200~400 nm

柱温30℃。结果: 毛蕊异黄酮苷、芒柄花素苷、毛蕊异黄酮、芒柄花素的线性范围分别为0.131 3~1.313 g·L-1(r=0.9997)

0.1186~1.186 g·L-1(r=0.9994)

0.020 6~0.206 g·L-1(r=0.9995)

0.015 0~0.150 g·L-1(r=0.999 5);平均回收率分别为97.07%

97.26%

97.45%

96.97%。结论: 本方法快速、准确、可靠

可以作为控制黄芪药材及制剂质量的方法。

Abstract

Objective: To establish UPLC method for the determination of main flavone in Astragalus membranaceus peparation. Method: ACQUITY UPLCTMBEH C18column(2.1 mm×50 mm

1.7 μm) was used with Methanol-0.2% formic acid Wateras a mobile phase. The flow rate was 0.5 mL·min-1

The detecting wavelength was set at 200-400 nm.The column temperature was at 30℃. Result: The linear ranges of calycosin glycoside

formononetin glycoside

calycosin and formononetin were 0.1313-1.313 g·L-1(r=0.9997)

0.1186-1.186 g·L-1(r=0.9994)

0.020 6-0.206 g·L-1(r=0.9995)

0.0150-0.150 g·L-1(r=0.9995). The average recoveries were 97.07%

97.26%

97.45%

96.97%. Conclusion: The method was reliable

accurate and quickly. It can be used for quality control of A. membranaceus peparation.

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Related Author

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Ye-wen FENG

Li ZHANG

Cheng-hao YU

BAI Yu-xin1

FU Liang2

Gao Jin2

XIA De-hong

Related Institution

Chengdu University of Traditional Chinese Medicine

Guangzhou University of Traditional Chinese Medicine

Southern Medicine University

College of Life Science and Agronomy,Shanxi Datong University

Jiangyin Hospital of Medical College.Southeast University,Jiangyin

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