Determination of Narigin, Hesperidin and Neohesperidin in Muxiang Fenqi Pill by HPLC

CHEN Xiao-ming; WANG Wei; MIN Qing-lu

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Determination of Narigin, Hesperidin and Neohesperidin in Muxiang Fenqi Pill by HPLC

更新时间：2024-01-04

- Determination of Narigin, Hesperidin and Neohesperidin in Muxiang Fenqi Pill by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 19, Pages: 115-117(2012)
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- Published：2012
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CHEN Xiao-ming, WANG Wei, MIN Qing-lu. Determination of Narigin, Hesperidin and Neohesperidin in Muxiang Fenqi Pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 115-117.
DOI：

CHEN Xiao-ming, WANG Wei, MIN Qing-lu. Determination of Narigin, Hesperidin and Neohesperidin in Muxiang Fenqi Pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 115-117. DOI：

摘要

目的:建立木香分气丸中柚皮苷、橙皮苷和新橙皮苷的含量测定方法。方法:采用反相HPLC

色谱柱为AgilentC18柱(4.6mm×250mm

5μm)

流动相乙腈-1%磷酸水(21:79)

流速1.0mL·min-1

检测波长283nm

柱温30℃。结果:柚皮苷进样量在0.1108~1.2188μg线性关系良好(r=0.9994

n=6)

平均回收率为97.5%(RSD0.80%

n=6);橙皮苷进样量在0.2268~2.4948μg线性关系良好(r=0.9997

n=6)

平均回收率99.0%(RSD1.7%

n=6);新橙皮苷进样量在0.0848~0.9328μg线性关系良好(r=0.9994

n=6)

平均回收率为98.2%(RSD1.6%

n=6)。结论:该方法重复性好、灵敏度高、定量准确

可作为木香分气丸质量控制方法之一。

Abstract

Objective:To develop an HPLC method for determination of narigin

hesperidin and neohesperidin in Muxiang Fenqi Pill. Method:The analysis was carried out with an Agilent C18(4.6 mm×250 mm

5μm)column and the mobile phase of acetonitrile-1% phosphoric acid (21:79).The flow rate was 1.0 mL·min-1 and column temperature was 30℃.The detection wavelength was set at 283 nm. Result:The calibration curve was linear within the range of 0.110 8-1.218 8 μg(r=0.999 4

n=6)

0.226 8-2.494 8 μg(r=0.999 7

n=6)and 0.084 8-0.932 8 μg(r=0.999 4

n=6) for narigin

hesperidin and neohesperidin.The average recoveries were 97.5%(RSD 0.80%

n=6)

99.0% (RSD 1.7%

n=6) and 98.2%(RSD 1.6%

n=6) respectively. Conclusion:The results showed this method is reproducible

sensitive and accurate for determination of narigin

hesperidin and neohesperidin in Muxiang Fenqi Pill.

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