Objective: To establish the method of specific chromatogram analysis of Rhei Radix Et Rhizoma by HPLC

and to study the correlation between Rhei Radix Et Rhizoma and its ethanol extract and to estimate the alcohol extracting technology. Method: HPLC was used with the Zorbax SB-Aq C18 column

the acetonitrile-phosphoric acid solution(φ=0.1%)(gradient elution) was employed as a mobile phase

detection wavelength was at 430 nm and 280 nm

column temperature was set at 40℃

and flow rate was 1.0 mL·min-1. Result: Fourteen common peaks were separated in the decoction pieces and its ethanol extract of Rhei Radix Et Rhizoma and 5 peaks were identified in 430 nm

the similarities of 8 batches of the decoction pieces and ethanol extract were over 0.96 and 0.95

the similarities of the other 2 batches were below 0.90.10 common peaks were separated in the decoction pieces and its ethanol extract and 2 peaks were identified in 280 nm

the similarities of 8 batches of the decoction pieces and ethanol extract were over 0.94 and 0.92

the similarities of the other 2 batches were below 0.90.The similarities between the decoction pieces and its ethanol extract were 0.992-0.999 in 430 nm and 0.987-0.999 in 280 nm

which respectively indicates that the traditional alcohol extracting technology of Rhei Radix Et Rhizoma can reserve the main characteristic component. Conclusion: The method is reliable

accurate and reproducible

which can be used for the quality control of the decoction pieces and ethanol extract of Rhei Radix Et Rhizoma as well as for compound preparation containing Rhei Radix Et Rhizoma.