Investigation of Solubility Parameter Sampling Method with Chromatographic Pharmacodynamics of Chinese Materia Medica
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Investigation of Solubility Parameter Sampling Method with Chromatographic Pharmacodynamics of Chinese Materia Medica
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 20, Pages: 6-11(2012)
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Published:2012
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LEI Hong, DUAN Xiao-peng, DENG Jun-lin, et al. Investigation of Solubility Parameter Sampling Method with Chromatographic Pharmacodynamics of Chinese Materia Medica[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(20): 6-11.
DOI:
LEI Hong, DUAN Xiao-peng, DENG Jun-lin, et al. Investigation of Solubility Parameter Sampling Method with Chromatographic Pharmacodynamics of Chinese Materia Medica[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(20): 6-11.DOI:
Investigation of Solubility Parameter Sampling Method with Chromatographic Pharmacodynamics of Chinese Materia Medica
Objective:To study dissolution regular pattern of ingredients from traditional Chinese medicine compound by solvent with different solubility parameter
and provide new ideas and theoretical basis for chromatographic pharmacodynamics sampling. Method: With different solubility parameters of solvent to dissolve Buyang Huanwu decoction extract
fingerprint chromatogram of ingredients from obtained lysate were determined by HPLC
and combining with total statistical moment theory
difference of solvent with different solubility parameter on component solution from total prescription was evaluated by total statistical moment similarity method. Result: RSD of AUCT
MCRTT
VCRTT from samples were 58.2%
12.8%
24.8%
respectively.It showed that chemical composition and total amount had significant difference
which was dissolved from solvent with different solubility parameter
its range of total statistical moment standard similarity 0.370-0.998.Sample similarity of S4
S5
S6 (solubility parameter of 18.33-20.60 J1/2·cm-3/2) compared to other samples
S7 and S8 (solubility parameter 21.73-22.87 J1/2·cm-3/2) compared to other samples except adjacent samples
these were less than 0.9
it indicated that constituent ratio of solvent on total prescription dissolution composition was different. Conclusion: Solubility parameter sampling method could make composition ratio of traditional Chinese medicine compound with significant difference
dissolution of solvent with different solubility parameter on total prescription had obvious law
it could provide an entirely new and referenced ideas for in vitro sampling with chromatographic pharmacodynamics
and could lay foundation for establishing a complete theoretical guidance of chromatographic pharmacodynamics sampling.
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Related Institution
School of Pharmacy,Hunan Key Laboratory of Druggability and Preparation Modification of Traditional Chinese Medicine,Laboratory of Supramolecular Mechanism and Mathematic-physics Characterization for Chinese Materia Medica,Hunan University of Chinese Medicine
College of Pharmacy,Hunan Key Laboratory of Druggability and Preparation Modification for Traditional Chinese Medicine(TCM),Property and Pharmacodaynamic Key Laboratory of TCM of National Administration of TCM,Hunan University of Chinese Medicine
The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People′s Hospital
Jilin Modern Chinese Medicine Engineering and Research Center Co. Ltd.
School of Pharmacy,Chengdu University of Traditional Chinese Medicine ( TCM )