Objective: To compare the sensitization effect of old Shengmai injection and new Shengmai injection on Beagle dogs. Method: Beagle dogs were randomly divided into 3 groups

3 dogs in each group. Each group was injected with 5% glucose injection

old Shengmai injection and new Shengmai injection. The changes of each dog were observed from injection before and after injection until 24 hours

and the response level were determined according to the severity of the symptoms. Blood samples were collected before injection and at 10 min after injection for measuring histamine content in plasma by ELISA. Sensitization of the injection was comprehensively determined by combined the response level of symptoms and the histamine level. Result: The old Shengmai injection led to beagle dogs showing typical symptoms of anaphylactoid reactions and elevating serum histamine times.The old Shengmai injection caused Beagle dogs anaphylactoid reactionis was positive. The new Shengmai injection led to Beagle dogs showing untypical symptoms of anaphylactoid reactions and elevating serum histamine times.It caused Beagle dogs anaphylactoid reaction was suspicious. Conclusion: The old Shengmai injection can induce typical anaphylactoid reactions on beagle dogs

and the sensitization is strong. Compared with the old Shengmai injection

the symptoms of anaphylactoid reactions induced by the new Shengmai injection appeared later and showed lesser degree. The sensitization to beagle dogs can bereduced.