Stability of Protein of Ethanol Extract from  with pH, Inorganic Salt and Organic Solvent

WU Shao-hui; LUO Ting-shun; ZHANG Cheng-gui; WU Xiu-mei; LIU Guang-ming

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Stability of Protein of Ethanol Extract from with pH, Inorganic Salt and Organic Solvent

更新时间：2024-01-04

- Stability of Protein of Ethanol Extract from with pH, Inorganic Salt and Organic Solvent
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 21, Pages: 74-77(2012)
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- Published：2012
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WU Shao-hui, LUO Ting-shun, ZHANG Cheng-gui, et al. Stability of Protein of Ethanol Extract from with pH, Inorganic Salt and Organic Solvent[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(21): 74-77.
DOI：

WU Shao-hui, LUO Ting-shun, ZHANG Cheng-gui, et al. Stability of Protein of Ethanol Extract from with pH, Inorganic Salt and Organic Solvent[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(21): 74-77. DOI：

摘要

目的: 对美洲大蠊醇提取物中蛋白质稳定性进行研究。方法: 通过采用UV法和Folin-酚试剂法

考察了pH、无机盐和有机溶剂等影响因素对蛋白质稳定性的影响。结果: pH、氯化钠、硫酸铵、乙腈和甲醇随着剂量的增加

在不同程度上破坏了蛋白质的结构。结论: 不同的影响因素通过改变蛋白质物理化学性质

破坏了蛋白质的稳定性。

Abstract

Objective: Studying the stability of the protein of atitumor activity from Periplaneta americana. Method: The factors of pH

inorganic salt and organic solvent on stability of the protein were studied by the UV and Lowrry method. Result: pH

sodium chloride

ammonium sulfate

acetonitrile and methanol are added and the structure of protein is changed. Conclusion: The physical and chemical properties are changed by different kinds of factors

and finally the stability of the protein is damaged.

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ZHANG Cheng-gui

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GAO Meng-ting

MA Fang-fang

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GENG Ling

LI Dong-mei

Related Institution

College of Pharmacy and Chemistry,Dali University,Dali

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