TNF-α Induced Insulin Resistance Model in 3T3-L1 Adipocyte

ZHANG Chang-hua; HU Xian-hong; ZHANG Zhong-wei; LIU Yu-hui; YU Ri-yue; XU Guo-liang

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TNF-α Induced Insulin Resistance Model in 3T3-L1 Adipocyte

更新时间：2024-09-23

- TNF-α Induced Insulin Resistance Model in 3T3-L1 Adipocyte
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 18, Pages: 171-174(2012)
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- Published：2012
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ZHANG Chang-hua, HU Xian-hong, ZHANG Zhong-wei, et al. TNF-α Induced Insulin Resistance Model in 3T3-L1 Adipocyte[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 171-174.
DOI：

ZHANG Chang-hua, HU Xian-hong, ZHANG Zhong-wei, et al. TNF-α Induced Insulin Resistance Model in 3T3-L1 Adipocyte[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 171-174. DOI：

摘要

目的: 应用肿瘤坏死因子α (TNF-α)诱导3T3-L1脂肪细胞

探讨建立可靠胰岛素抵抗(IR)细胞模型的方法。方法: 3T3-L1前脂肪细胞经3-异丁基-1-甲基黄嘌呤、地塞米松、胰岛素诱导分化成3T3-L1脂肪细胞

将其与20

5 μg ·L-1TNF-α共孵育

100 nmol ·L-1胰岛素作用30 min刺激脂肪细胞糖转运。以葡萄糖氧化酶法测定培养基上清液葡萄糖含量

观察TNF-α对脂肪细胞糖摄取的影响

鉴定IR模型。结果: TNF-α抑制胰岛素诱导前、后的脂肪细胞糖转运

抑制作用呈剂量依赖性

其中20 μg ·L-1 TNF-α的抑制率分别为79.2%和81.4%(P<0.05)。结论: 肿瘤坏死因子α可诱导3T3-L1脂肪细胞产生IR

这种细胞模型简便、可靠。

Abstract

Objective: To study the effect of tumor necrosis factor α(TNF-α) on glucose consumption in 3T3-L1 adipocyte and find the method of establishing a cell insulin resistance model. Method: The 3T3-L1 preadipocytes were differentiated to 3T3-L1 adipocytes with 1-methyl-3-isobuthylxanthine

dexamethasone and insulin. 3T3-L1 adipocytes were exposed to 20

5 μg · L-1 TNF-α for 96 h

then added 100 nmol · L-1 insulin for 30 min. The amount of glucose consumption was determined by detecting the glucose content in cell culture supernatants with glucose oxidase(GOD) assay. Result: TNF-α markedly decreased both basal and insulin-stimulated glucose uptake.This effect was concentration-dependent

and 20 μg · L-1TNF-α decreased glucose uptake of 79.2% and 81.4% (P<0.05) in the basal and 100 μg · L-1 insulin-stimulated conditions

respectively. Conclusion: 20 μg · L-1TNF-α can induce insulin resistance in 3T3-L1 adipocytes.A novel and reliable cell model of insulin resistance is successfully established.

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Related Author

ZHANG Chang-hua1

HU Xian-hong1

ZHANG Zhong-wei2

LIU Yu-hui1

YU Ri-yue1

XU Guo-liang2

WANG Chun-yi

LI Wei-min

Related Institution

Department of Pharmacology,College of Pharmacy,Jiangxi University of Traditional Chinese Medicine

中山大学中山医学院药理学教研室

江西中医学院现代中药制剂教育部重点实验室

Guang'anmen Hospital，China Academy of Chinese Medicine Sciences

First Teaching Hospital of Tianjin University of Traditional Chinese Medicine（TCM）

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