Effect of Andrographolide on Vasoactive Substances in Myocardial Hypertrophy Rats

HUANG Zhi-hua; ZENG Xue-liang; QIU Li-li; XIONG Dong-lian; ZENG Jing; HAN Li-min

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Effect of Andrographolide on Vasoactive Substances in Myocardial Hypertrophy Rats

更新时间：2024-01-04

- Effect of Andrographolide on Vasoactive Substances in Myocardial Hypertrophy Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 12, Pages: 166-169(2012)
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- Published：2012
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HUANG Zhi-hua, ZENG Xue-liang, QIU Li-li, et al. Effect of Andrographolide on Vasoactive Substances in Myocardial Hypertrophy Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(12): 166-169.
DOI：

HUANG Zhi-hua, ZENG Xue-liang, QIU Li-li, et al. Effect of Andrographolide on Vasoactive Substances in Myocardial Hypertrophy Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(12): 166-169. DOI：

摘要

目的: 观察穿心莲内酯(andrographolide

AP)对异丙肾上腺素(isopropylarterenol

Iso)所致大鼠心肌肥厚的保护作用及其对血管紧张素Ⅱ(angiotensin Ⅱ

AngⅡ)、心钠素(atrial natriuretic peptide

ANP)、内皮素(endothelin

ET)和一氧化氮(nitric oxide

NO)的影响。方法: 以Iso 1 mg·kg-1背部皮下注射(sc)连续10 d建立大鼠心肌肥厚模型。选模第2天给药组 sc给予低、中、高剂量(0.1

0.3

1.0 μmol·kg-1)的AP

溶剂组sc二甲基亚砜

正常组和模型组sc给予生理盐水

连续14 d

末次给药后禁食12 h

取血

分离血清及血浆

取心肌组织并制成10%匀浆。酶活性分析法检测血清及心肌组织乳酸脱氢酶(lactate dehydrogenase

LDH)活性;硝酸还原酶法检测血清NO含量;放射免疫法测定血浆AngⅡ

ANP和ET的含量。结果: 与正常对照组相比

模型组大鼠血清LDH活性升高(1 425.4±304.7)U·mL-1

(P<0.01)

心肌组织LDH活性降低(662.5±172.6)U·g-1

(P<0.05)

血浆ET(62.45±12.27) ng·L-1(P<0.05)

含量升高

ANP(241.82±127.71)ng·g-1

(P<0.05)和NO(37.21±2.62)μmol·L-1(P<0.01)含量降低。与模型组相比

AP处理后血清LDH活性降低(P<0.001)

组织LDH活性升高(P<0.05)

血浆AngⅡ及ET含量下降(P<0.05)

而血浆ANP含量及血清NO含量升高(P<0.05)。结论: 穿心莲内酯可能通过影响大鼠血管活性物质的平衡

抑制RAS系统及ET的合成

增加ANP及NO的合成

从而抑制心肌肥大及心力衰竭的进程

对心脏起保护作用。

Abstract

Objective: To observe the protection of andrographolide (AP) on rat's myocardial hypertrophy induced by isopropylarterenol (Iso)

explore balance of vasoactive substance. Method: The rat's myocardial hypertrophy model is formed through hypodermic injection which extended 10 days at back skin with Iso

1 mg·kg-1·d-1. Once the model is formed

it will be administrated with various doses of AP

dimethyl sulfoxide(DMSO) or normal saline through hypodermic injection from the second day

and the process will extend 14 days. After the last administration

the rat will be in absolute diet status for 12 h and its blood and heart are taken under anesthesia. The activity of lactate dehydrogenase (LDH) in serum and myocardium tissue

the level of NO in serum

and the content of angiotensin Ⅱ(AngⅡ)

endothelin(ET) and atrial natriuretic peptide (ANP)in plasma were investigated. Result: Compared with control group

Iso group showed a change about activity of LDH which was increased in serum but decreased in myocardium tissue

a descended level of NO in serum

improved content of AngⅡ and ET while reduced content of ANP in plasma. However

the activity of LDH is depressed in serum and elevated in myocardium tissue in AP groups

furthermore

the level of NO and ANP were enhanced while the content of AngⅡ and ET were reduced in AP groups. Conclusion: AP has a protection on rat's myocardial hypertrophy caused by Iso

and its mechanism probably result of modulating the homeostasis of AngⅡ

ANP

ET and NO.

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Related Institution

First Affiliated Hospital,Gannan Medical University

Gannan Medical University

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