HPLC Determination of Three Components in Fufang Tujingpiding

LI Xiao-cui; MIAO Ai-dong

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HPLC Determination of Three Components in Fufang Tujingpiding

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- HPLC Determination of Three Components in Fufang Tujingpiding
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 19, Pages: 108-111(2012)
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- Published：2012
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LI Xiao-cui, MIAO Ai-dong. HPLC Determination of Three Components in Fufang Tujingpiding[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 108-111.
DOI：

LI Xiao-cui, MIAO Ai-dong. HPLC Determination of Three Components in Fufang Tujingpiding[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(19): 108-111. DOI：

摘要

目的:建立高效液相色谱法测定复方土荆皮酊中土荆皮乙酸、苯甲酸和水杨酸3种成分含量的方法。方法:采用高效液相色谱法

以AgilentZorbaxSBC18(4.6mm×150mm

5μm)为色谱柱

流动相为甲醇-0.09%甲酸(58:42)

流速1.0mL·min-1

柱温25℃

检测波长260nm(检测土荆皮乙酸)和230nm(检测苯甲酸和水杨酸)。结果:土荆皮乙酸、苯甲酸和水杨酸分别在1.29~25.78mg·L-1(r=0.9998)

0.022~0.266g·L-1(r=0.9999)

0.011~0.110g·L-1(r=0.9998)与峰面积呈良好的线性关系;土荆皮乙酸、苯甲酸和水杨酸的平均加样回收率(n=6)分别为100.4%

100.9%

100.5%;RSD分别是1.00%

0.84%

0.75%。结论:该方法准确、简便、重复性好

可用于复方土荆皮酊的质量控制。

Abstract

Objective:To establish an HPLC method for the content determination of pseudolaric acid B

benzoic acid and salicylic acid in Fufang Tujingpiding. Method:The analysis was performed on the Agilent Zorbax SB C18(4.6 mm×150 mm

5 μm) column with the methol-0.09% formic acid as the mobile phase at 25℃. The flow rate was 1.0 mL·min-1

and the detection wavelength was set at 260 nm for pseudolaric acid B

230 nm for benzoic acid and salicylic acid. Result:The calibration curves were linear in the ranges of 1.29-25.78 mg·L-1(r=0.999 8) for pseudolaric acid B

0.022-0.266 g·L-1(r=0.9999) for benzoic acid and 0.011-0.110 g·L-1(r=0.999 8)for salicylic acid. The average recoveries (n=6) of pseudolaric acid B

benzoic acid and salicylic acid were 100.4%(RSD 1.00%)

100.9% (RSD 0.84%) and 100.5% (RSD 0.75%)

respectively. Conclusion:The developed method is accurate

convenient with good reproducibility that can be applied to the quality control of Fufang Tujingpiding.

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Related Institution

School of Life Science, Huaibei Normal University, Anhui Key Laboratory of Plant Resources and Biology

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