Content Determination of Matrine in A'naer Vigills by RP-HPLC
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Content Determination of Matrine in A'naer Vigills by RP-HPLC
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 14, Pages: 119-121(2012)
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Published:2012
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SHI Yin-ji, LIU Di-wei, SHI Xue, et al. Content Determination of Matrine in A'naer Vigills by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(14): 119-121.
DOI:
SHI Yin-ji, LIU Di-wei, SHI Xue, et al. Content Determination of Matrine in A'naer Vigills by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(14): 119-121.DOI:
Content Determination of Matrine in A'naer Vigills by RP-HPLC
Objective: To develop an RP-HPLC method for the determination of matrine content in A’naer vigills. Method: By RP-HPLC
Kromasil (4.6 mm×250 mm
5 μm) column was used with mobile phase consisted of methanol-0.04% triethylamine (61∶39) and flow rate set at 0.7 mL·min-1. The detection wavelength was set at 220 nm and column temperature was set at 25 ℃. The injection volume was 10 μL. Result: The linear response range of matrine was 19.12-153.0 mg·L-1 (r=0.999 8). The average recovery was 95.17%
RSD was 1.11% (n=9). The product in the 12 h solution in stability. Conclusion: The method is simple
reliable
sensitive
accurate and reproducible for quality control of A’naer vigills.
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