Objective: To study the protective effect of the Chrysanthemum indicum polysaccharide(CIP) on immunosuppressed mice caused by cyclophosphamide. Method: The mice were randomly divided into four groups (10 in each group): normal control group

model group and CIP groups (high and low dosage). CIP high and low dosage groups were administered intragastrically with CIP at dose of 400

200 mg·kg-1

respectively once daily for fourteen days. At the tenth day model group and CIP groups were administered with cyclophosphamide by peritoneal injection for four days to induce an immunosuppressed mouse model. The effect on carbon clearance was observed. The weight of the mice

thymus and spleen was measured; the thymus index and spleen index were calculated. The cell immune function was assessed by delayed type hypersensitivity induced by dinitrofluorobenzene in normal mice. The serum level of hemolysin was determined. Result: All the indexes in the model group were reduced(P<0.01). The carbon clearance rate in CIP high and low dosage groups was 0.0278±0.0059 and 0.0199±0.0047 respectively

the phagocytic exponent was 5.3970±0.7356 and 4.8278±0.4801

ear edema was (5.23±0.98) mg and (4.89±1.10) mg

the serum level of hemolysin was 0.410±0.063 and 0.357±0.058. The carbon clearance rate in model group was 0.0134±0.0038

the phagocytic exponent was 4.2316±0.3672

ear edema was (3.21±0.91)mg

the serum level of hemolysin was 0.299±0.032.Compared with model group

all was significantly increased (P<0.01 orP<0.05). Conclusion: CIP can improve the non-specific

humoral and cellular immune functions in cyclophosphamide-induced immunosuppressed mice.