Determination of Contents of Total Flavonoids in  by UV

CHEN Jun; WEI Jian-hua; LI Bing; CAI Shao-fang; ZHANG Ming-zhan

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Determination of Contents of Total Flavonoids in by UV

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- Determination of Contents of Total Flavonoids in by UV
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 13, Pages: 105-107(2012)
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- Published：2012
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CHEN Jun, WEI Jian-hua, LI Bing, et al. Determination of Contents of Total Flavonoids in by UV[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 105-107.
DOI：

CHEN Jun, WEI Jian-hua, LI Bing, et al. Determination of Contents of Total Flavonoids in by UV[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 105-107. DOI：

摘要

目的: 建立紫外分光光度法测定广西不同产地倒地铃全草总黄酮的含量。方法: 以芦丁为对照品

以亚硝酸钠-硝酸铝-氢氧化钠为显色剂

测定波长为488 nm。结果: 芦丁在0.00935~0.0560 g·L-1与峰面积具有良好的线性关系(r=0.9993);平均加样回收率为100.05%(n=6)

RSD 2.3%。结论: 该法准确、简便、稳定可靠

可用于倒地铃中总黄酮的含量测定。

Abstract

Objective: To establish the methods of determination on the contents of total flavonoids in Cardiospermum halicacabumby UV. Method: Rutin was used as reference substance and sodium nitrite-nitrate aluminum-sodium hydroxide as color-showing reagent

measurement wavelength was set at 488 nm. Result: The linear response range was 0.00935-0.0560 g·L-1(r=0.9993). The average recovery was 100.05%

RSD 2.3%(n=6)。 Conclusion: The method was simple

accurate and repeoducible which could be used for determination of the total flavonoids in C. halicacabum.

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Related Author

Tao LIU

Chun-mei FU

Yu TANG

Jia ZHANG

Zhen-xiang HE

Jie YU

ZHANG Mei-ling1

ZHEN Han-shen2

Related Institution

Chengdu University

Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences and Peking Union Medical College

School of Life Sciences， Tsinghua University

Center of Pharmacy R&D，XinJiang Huachun Bio-Pharmaceutical Co. Ltd.

Pharmacy Technology Center，Tsinghua University

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