Objective: To observe the effect of Chaipu decoction on expression of Bcl-2 in lung tissue in asthma guinea pigs. Method: Forty guinea pigs were randomized into normal control

model

dexamethasone (0.001 g·kg-1) and chaipu decoction (0.35 g·kg-1) groups with 10 guinea pigs each group. Asthma mouse model was prepared by injecting ovalbumin (OVA) into the abdominal cavity and inhalation of aerosol

and normal group

was given saline water. Each group was given relevant medicines by oral administration before 30 mins of suscitation corresponding

once every other day

for 2 weeks. All animals were sacrificed after the last stimulation. The white blood cell(WBC) count

the number of eosinophil(EOS) and the percentage of EOS in bronchoalveolar lavage fluid (BALF) of guinea pigs and the number of EOS in whole blood were examined. Pulmonary pathological changes were observed by HE stain. Bcl-2 protein expression was examined by immunohistochemistry. And Bcl-2 mRNA expression was detected with in situ hybridization. Result: Compared with model group

chaipu decoction group could significantly reduce the EOS count in whole blood

the WBC count

the number of EOS and the percentage of EOS in BALF(P<0.05); and the expression level of Bcl-2 mRNA and protein in the lung tissue were significantly decreased(P<0.05). In Chaipu decoction group

compared with dexamethasone group

the significant difference was not found. Conclusion: Chaipu decoction for asthma was able to reduce the proportion of EOS in BALF

alleviate the inflammation of airway and lung tissue of the asthmatic guinea pigs. Chaipu decoction for asthma could reduce the Bcl-2 mRNA and protein expression level.