Objective: To investigate the preparation progresses of muti-herbs remedy

based on pharmacokinetic theory

taken Huanglian Jiedu decoction (HLJDT) example. Method: The plasma samples were collected at different time points

after oral administration of products of HLJDT by four different preparation methods

RP-HPLC was used to determine plasma levels of geniposide which was an index component and higher levels of HLJDT

then concentration-time curve was drawn. And DAS 2.0 software was used to simulate the corresponding pharmacokinetic parameters. Result: Specificity and accuracy of the method were in line with the requirements of biological sample analysis. The analytical results showed that plasma concentration was a larger difference between the oral products made from the same formula HLJDT

but prepared in a different way. The AUC of sediment group of aqueous extract of the multi-herbs remedy (Ⅲ) was minimum and elimination of that was fastest; there was a significant difference comparing the AUC(0~∞)between aqueous extract of the multi-herbs remedy without sediment (Ⅱ) and aqueous extract of the multi-herbs remedy (Ⅰ) and the whole side of water extraction and alcohol precipitation refined extract group (Ⅳ) (P<0.05); the concentration-time curve of geniposide in sample Ⅳ showed flat trend; the Cmax of geniposide in aqueous extract of the multi-herbs remedy was the largest

but elimination of it was more faster than that of sample Ⅳ. Conclusion: comparing the pharmacokinetics of the four products prepared by different ways

prompted that the preparation processes of multi-herbs remedy on pharmacokinetics studies could be based on the demands of clinical drug formulations and that of dosing interval

which preliminarily provided a new idea to evaluate preparation processes of multi-herbs remedy.