Objective: To analyze transdermal effect and transdermal composition of volatile oil from Notopterygium incisum

Angelica pubescens and pair of them by GC-MS. Method: Chemical constituents of volatile oil in in vitro transdermal donor cell and receiving cell were identified by GC-MS

The relative contents in percentage were calculated with area normalization method.According to mathematical derivation

relative contents was changed into translation rate

which reflected transdermal effect of every components. Result: After percutaneous absorption

thirty-nine components were identified in N. incisum (93.22%)

twenty-nine in A. pubescens (93.74%) and thirty-six in drug pair of them (92.75%).Main components of percutaneous absorption in N. incisum were 1-terpineol (30.96%)

4-terpineol (28.22%)

borneol(7.10%)

etc;Main components of percutaneous absorption in A. pubescens were o-acetyl-p-methylbenzyl alcohol (55.70%)

trans-oxy-linalool (6.79%)

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7-trimethyl-bicyclic 5-hexene-2-one(4.78%)

etc;Main components of percutaneous absorption in drug pair of them were 1-terpineol (28.86%)

4-terpineol (22.41%)

o-acetyl-p-methylbenzyl alcohol (12.15%)

borneol (5.62%)

etc. Conclusion: Transmission rate of every components in three kinds of volatile oil could be sorted qualitatively of transdermal effect.Transmission rate of 1-terpineol was the most in volatile oil of N. incisum. 1

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7-trimethyl-bicyclic 5-hexene-2-one had the most transmission rate in volatile oil of A. pubescens. 1-terpineol had the most transmission ratio in volatile oil of drug pair of them.