Optimization of Purification Process for Total Alkaloids from by Macroporous Resin
|更新时间:2024-01-04
|
Optimization of Purification Process for Total Alkaloids from by Macroporous Resin
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 14, Pages: 18-22(2012)
作者机构:
作者简介:
基金信息:
DOI:
CLC:
Published:2012
稿件说明:
移动端阅览
DONG Jie, ZHU Hui-min, HU Kai-wen, et al. Optimization of Purification Process for Total Alkaloids from by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(14): 18-22.
DOI:
DONG Jie, ZHU Hui-min, HU Kai-wen, et al. Optimization of Purification Process for Total Alkaloids from by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(14): 18-22.DOI:
Optimization of Purification Process for Total Alkaloids from by Macroporous Resin
Objective: To optimize purification process of total alkaloids from Fritillaria thunbergii by macroporous resin. Method: Optimum macroporous resin and its purification process was selected by static and dynamic adsorption and desorption kinetics test. Result: Optimal purification process conditions were:optimal macroporous resin type HPD100
diameter-height ratio 1∶9
the concentration of sample solution 5 g·mL-1 with pH as 8.0
maximum sample volume 13 BV
flow rate of adsorption and elution 1 mL·min-1
washed by 8 BV water and 10 BV 80% ethanol
collected eluent of 80% ethanol.Under this optimized technology
the content of total alkaloids was more than 65% by UV. Conclusion: HPD100 type macroporous resin showed good comprehensive adsorption property for enrichment of total alkaloids from F. thunbergii
and suitable for separation and purification of it.
Purification Process of Total Alkaloids from by Macroporous Resin
Optimization of Purification Process of Total Alkaloid Extract of Berberis dictyophylla Cortex by Macroporous Resin and Investigation of Its Quality Standard
Studies on Purification of Saponins (PNS) with Macroporous Resin
Study on Separation and Purification Total Glycosides of Tree Peony Bark by Macroporous Resin
Related Author
MENG Jiang
LIANG Hui-chao
CHEN Lei
LU Guo-yong
WU Dan-feng
Hui FENG
Ya ZHAO
Xiao-yan WANG
Related Institution
Ethnic Medicine School, Chengdu University of Traditional Chinese Medicine
Tibetan Medical College, Qinghai University
School of Pharmacy,Shandong University of Traditional Chinese Medicine,Ji’nan
Zhejiang Chinese Medical University
School of Pharmacy,Tianjin University of Traditional Chinese Medicine