Objective: To observe the effect of Dryopteris laeta polysaccharide on α-glucosidase enzyme and blood glucose in mice. Method :The α-glycosidase enzymes activity determination method was used to determine the polysaccharide dryopteris beautiful plants for α-glucosidase inhibitory activity; D. laeta polysaccharide was considered as experimental drugs

the mice with blood glucose range of 4.0-9.0 mmol·L-1

were chosen. According to the weight mice were divided in at random into 16 groups

10 each group. After 3 days of adaptive feeding

mice were given with normal saline (0.135 g·kg-1)

acarbose(0.625 g·kg-1)

high doses of crude polysaccharide(0.48 g·kg-1)

low dose of crude polysaccharide (0.24 g·kg-1)

once a day. After 4 days of modeling

at day 5 mice were fed with glucose (2.0 g·kg-1)

sucrose (4.0 g·kg-1)

maltose (4.0 g·kg-1)

starch (6.0 g·kg-1) as a loading dose. In 15

30

60

120 min

blood glucose was measured

the influence of D. laeta polysaccharide on glucose

maltose

starch loading doses of blood glucose was observed after feeding sucrose in normal mice. Result :When D.laeta polysaccharide concentration in the sample was under 10 g·L-1

reaction of α-glucosidase inhibitory activity reached more than 90.8%

which was considered as a competitive type of inhibition (Ki=2.53 g·L-1). The polysaccharide as the experimental drug

after giving a loading dose of starch sugar

compared with control group

blood glucoselevel in maltose and starch group decreased more significantly (P<0.01)

followed by sucrose group (P<0.05). Conclusion: D.laeta polysaccharide has an inhibitory activity on α-glucosidase

which is considered as a competitive inhibition

and can improve glucose tolerance in normal mice.