Study on Chromatography Fingerprint of Radix Astragali with Hyphenated Technique of HPLC-DAD-ELSD
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Study on Chromatography Fingerprint of Radix Astragali with Hyphenated Technique of HPLC-DAD-ELSD
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 17, Pages: 70-74(2012)
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Published:2012
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LIANG Jin, LIU Xiao-hua, REN Yuan, et al. Study on Chromatography Fingerprint of Radix Astragali with Hyphenated Technique of HPLC-DAD-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(17): 70-74.
DOI:
LIANG Jin, LIU Xiao-hua, REN Yuan, et al. Study on Chromatography Fingerprint of Radix Astragali with Hyphenated Technique of HPLC-DAD-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(17): 70-74.DOI:
Study on Chromatography Fingerprint of Radix Astragali with Hyphenated Technique of HPLC-DAD-ELSD
目的: 采用高效液相色谱法-二极管阵列检测器-蒸发光检测器 (HPLC-DAD-ELSD)联用技术对黄芪药材进行指纹图谱研究。 方法: 采用Waters 2695-SpursilTM C18色谱柱(4.6 mm× 250 mm
5 μm)
流动相乙腈-水梯度洗脱
流速1.0 mL·min-1
柱温25 ℃
进样量20 μL
检测波长254 nm。蒸发光散射检测器的条件为:漂移管温度112.8 ℃
载气流速3.2 L·min-1。 结果: 10批药材HPLC-DAD指纹图谱找到14个共有峰
鉴别了毛蕊异黄酮和芒柄花素;HPLC-ELSD指纹图谱找到9个共有峰
鉴别了毛蕊异黄酮苷、黄芪甲苷、黄芪皂苷Ⅲ、黄芪皂苷Ⅱ。 结论: 方法准确可靠
为全面控制黄芪药材质量提供了一种方法。
Abstract
Objective: To research the chromatography fingerprint of Radix Astragali with hyphenated technique of HPLC-DAD-ELSD. Method: The chromatography conditions were defined as SpursilTM C18 column (4.6 mm× 250 mm
5 μm); mobile phase was A:ACN
B:H2O
gradient elution; flow speed was 1.0 mL·min-1; temperature of column was set at 25 ℃; injection volume was 20 μL; detected wavelength was at 254 nm. The ELSD conditions were as follows: the temperature of drift tube was 112.8 ℃
the gas speed was 3.2 L·min-1. Result: In the chromatography fingerprint with HPLC-DAD of Radix Astragali
14 common peaks were demarcated
calycosin and formononetin were identified; in the chromatography fingerprint with HPLC-ELSD of Radix Astragali
9 common peaks were demarcated
calycosin-7-O-β-D-glucoside
astragaloside Ⅳ
astragaloside Ⅲ and astragaloside Ⅱ were identified. Conclusion: The method was accurate and reliable and the study may contribute in controlling of Radix Astragali.