Objective: Study on toxicity attenuation and efficacy reservation of Manchurian Dutchmanspipe Stem following five different processing methods

and to collect best processing methods. Method: Five methods of processing herbs with vinegar

honey

ginger

alkali and salt were compared focusing on the optimal toxicity attenuation effect with content of aristolochic acid

hederagenin and oleanolic acid (OA) from Manchurian Dutchmanspipe Stem as evaluated index detected by RP-HPLC. Result: Content of aristolochic acid in raw herbs and the herbs processed with vinegar

honey

ginger

alkali and salt was as follows: 0.27%

0.12%

0.15%

0.20%

0.14%

0.18%; content of oleanolic acid (OA) in raw herbs and the herbs processed with vinegar

honey

ginger

alkali and salt was: 0.21%

0.15%

0.20%

0.13%

0.01%

0.01%; content of hederagenin in raw herbs and the herbs processed with vinegar

honey

ginger

alkali and salt was: 0.11%

0.08%

0.09%

0.07%

0.01%

0.01% respectively. Conclusion: The honey processing was regarded as the optimal processing method for Manchurian Dutchmanspipe Stem.