Objective: Fractionation of ginseng polysaccharides were done by column chromatographic

and study the stimulation of collected fractions on proliferation of spleen lymphocyte to find out the active fractions. Method: Fractionation of ginseng polysaccharides was done by Q-Sepharose FF

separated GP into neutral polysaccharide and acidic polysaccharide with water and 0.4 mol·L-1 NaCl as eluents

then separated GPA into several fractions with 0-0.4 mol·L-1 NaCl as eluents. Spleen lymphocytes of mice was isolated and adjusted the spleen lymphocytes concentration to 3.0×106/mL

added sample and incubated for 48 h

the stimulation of each fractions with different concentration(25

50

100

200

400 mg·L-1)on proliferation of spleen lymphocyte was observed. Result: Obtained GPN and GPA from GP

separate GPA into GPA-1

GPA-2

GPA-3 and GPA-4.GP

GPA

GPN

GPA-2

GPA-3 and GPA-4 had the promoting function on proliferation of spleen lymphocyte

their biggest stimulating indexes were 1.073

1.082

1.119

1.101

1.102 and 1.296. The effects of GPA

GPA-2

GPA-3 and GPA-4 enhanced along with the increasing of concentration;with the increasing of concentration

the promoting action of GPN was reduced

when the concentration was over 100 mg·L-1

inhibition was found

and with the increasing of concentration

the inhibition became stronger. GPA-1 had suppression inhibitory effects on proliferation of spleen lymphocyte

and with the increasing of concentration

the inhibition became stronger. Conclusion: Different polysaccharide acid were separated from GP with Q-Sepharose FF. Fractionations of ginseng polysaccharides have different promoting function on proliferation of spleen lymphocyte

and GPA-4 had the strongest effect.