Objective: CO2 supercritical fluid extraction and decoction methods were used to extract Crocodylus siamensis oil

respectively. The oil yield

fatty acid yield and main oil compositions were set to be the investigation index. Method: C. siamensis oil was extracted by CO2 supercritical fluid extraction and decoction methods respectively. The oil yield and fatty acid yield were compared. The relative percentage of the components was determined by the area normalization method using gas chromatography (GC)/electron impact (EI) mass spectrometric (MS). Result: The oil yields were 32.6%

60%

respectively

while the fatty acid yields were 73.06%

76.78%. Mainly compositions extracted by the two methods were not identical. Conclusion: Decoction method has certain advantage over CO2 supercritical fluid extraction method at the aspect of oil yield. The way of extracting C. siamensis oil by CO2 supercritical fluid extraction method still needs further study and optimization.