Determination of Bufothionine in Different Parts from Two Species of Toad Skin Using HPLC

WANG Hong-jie; ZHAO Hai-yu; SI Nan; BIAN Bao-lin; YANG Li-xin

您当前的位置：

首页 >

文章列表页 >

Determination of Bufothionine in Different Parts from Two Species of Toad Skin Using HPLC

更新时间：2024-01-04

- Determination of Bufothionine in Different Parts from Two Species of Toad Skin Using HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 11, Pages: 66-68(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2012
- 稿件说明：
移动端阅览
WANG Hong-jie, ZHAO Hai-yu, SI Nan, et al. Determination of Bufothionine in Different Parts from Two Species of Toad Skin Using HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 66-68.
DOI：

WANG Hong-jie, ZHAO Hai-yu, SI Nan, et al. Determination of Bufothionine in Different Parts from Two Species of Toad Skin Using HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 66-68. DOI：

摘要

目的: 对不同种蟾皮不同部位中蟾蜍噻咛的含量进行了比较研究。方法 :采用高效液相色谱法测定了不同种蟾皮中不同部位蟾蜍噻咛的含量。样品中蟾蜍噻咛峰通过与对照品进行HPLC-DAD 3D及HPLC-MS-MS比对分析进行了确定。提取方法通过正交试验确定为50倍量50%甲醇超声60 min。样品分析选用了WondsilTM C18 色谱柱(4.6 mm×250 mm

5 μm)

以乙腈-水(6 :94)为流动相

检测波长226 nm。结果 :该方法具有良好的线性(r=0.999 7)、精密度(RSD

0.59%)、稳定性(RSD

0.72%)和重复性(RSD

1.56%);平均加样回收率为98.57%

RSD为2.03%

表明该方法准确可靠。经测定

中华大蟾蜍与黑框蟾蜍不同部位均含有蟾蜍噻咛成分

含量为0.07%~0.17%。结论 :蟾蜍噻咛在不同种蟾皮药材中的不同部位含量差异不大。

Abstract

Objective: To determine bufothionine in different parts of the dried toad skin from two species. Method: A quantification method was developed by HPLC with photodiode array (PDA) detection. Bufothionine was identified unambiguously by HPLC-DAD 3D and HPLC-MS-MS analysis in comparison with reference standard. The extraction method was optimized as 50 times 50% MeOH ultrasonic 60 min. The analysis was performed on WondsilTM C18 column (4.6 mm×250 mm

5 μm) with ACN-H2O (6 : 94) as mobile phase and detection wavelength was at 226 nm. Result : The samples were analyzed with good linear regression relationship (r=0.999 7)

precisions (RSD

0.59%)

repeatability (RSD

1.56%)

stability (RSD

0.73%) and recovery (98.57%; RSD

2.03%). Bufothionine was detected in different parts of toad skin from Bufobufo gargarizans cantor and Bufo melanostictus Schneider. The contents were from 0.07% to 0.17%. Conclusion : The content of bufothionine revealed no significant differences among the different parts in these two species of toad skin.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

No data

Related Author

No data

Related Institution

No data

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰