Determination of Total Polyphenol Content in Different Parts of Rhizoma Bletillae Striatae

ZHOU Yun-kai; LI Wei-ping; TIAN Sha-sha; MA Dan-dan; JIANG Fu-sheng; DING Zhi-shan

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Determination of Total Polyphenol Content in Different Parts of Rhizoma Bletillae Striatae

更新时间：2024-01-04

- Determination of Total Polyphenol Content in Different Parts of Rhizoma Bletillae Striatae
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 10, Pages: 161-164(2012)
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- Published：2012
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ZHOU Yun-kai, LI Wei-ping, TIAN Sha-sha, et al. Determination of Total Polyphenol Content in Different Parts of Rhizoma Bletillae Striatae[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 161-164.
DOI：

ZHOU Yun-kai, LI Wei-ping, TIAN Sha-sha, et al. Determination of Total Polyphenol Content in Different Parts of Rhizoma Bletillae Striatae[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 161-164. DOI：

摘要

目的: 测定白及块茎和须根中总酚的含量。方法: 以没食子酸为对照品

采用folin-酚比色法测定白及块茎和须根中的总酚含量。采用TLC对白及须根和块茎进行定性比较。结果: 采用folin-酚比色法测定白及中总酚含量

准确度好

精密度高。没食子酸对照品在5~25 μg的范围内线性良好

标准曲线方程为Y=0.033 2X+0.000 1。白及须根中的总酚含量为0.895%

块茎中的总酚含量为0.394%。TLC结果表明

白及须根的斑点比块茎多。结论: 白及须根中总酚含量高于块茎。

Abstract

Objective: To determine the total polyphenol content in the fibrous roots and tubers of Rhizoma Bletillae Striatae. Method: The content of total polyphenol in the fibrous roots and tubers of Rhizoma Bletillae Striatae was detected by folin colorimetry method using gallic acid as index

then using TLC method to qualitative compare fibrous roots with tubers of Rhizoma Bletillae Striatae. Result: There was a good linearity in the range of 5-25 μg for the gallic acid

the standard curve equation was Y=0.033 2X+0.000 1. The total polyphenol content in the fibrous roots was 0.895% while tubers was 0.394%. Conclusion: the total Polyphenol content in the fibrous roots of Rhizoma Bletillae Striatae is higher then tuber.

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Related Institution

Zhejiang Chinese Medical University

贵州师范大学天然药物质量控制研究中心,贵州师范学院药用植物研究所,贵州师范大学天然药物质量控制研究中心,贵州师范学院药用植物研究所

贵州师范学院药用植物研究所

Hubei Institute for Food and Drug Control

白求恩医科大学制药厂

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