Objective: To determination of jatrorrhizine

epiberberine

coptisine

palmatine and berberine hydrochloride in purification parts of ‘Sanrelun’ compound. Method: ‘Sanrelun’ compound through HPD 400 macroporous resin with 10%

30%

60%

90% ethanol eluted

then was determinated the five alkaloids in four purification parts by HPLC. Result: The highest content of alkaloids was the part of 30%EtOH purified by HPD 400 macroporous resin. Conclusion: In this study

the method of macroporous resin purification is simple

alkaloid content which was determinated by HPLC is highest in the part of 30% of the purified

which provide the foundation for effective parts selection.