Content Determination of Total Polysaccharide in the Garlic Polysaccharide by Colorimetry
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Content Determination of Total Polysaccharide in the Garlic Polysaccharide by Colorimetry
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 10, Pages: 158-161(2012)
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Published:2012
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WANG Rui-hai, BAI Dong, LIU Li-mei. Content Determination of Total Polysaccharide in the Garlic Polysaccharide by Colorimetry[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 158-161.
DOI:
WANG Rui-hai, BAI Dong, LIU Li-mei. Content Determination of Total Polysaccharide in the Garlic Polysaccharide by Colorimetry[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(10): 158-161.DOI:
Content Determination of Total Polysaccharide in the Garlic Polysaccharide by Colorimetry
Objective: To develop a method for content determination of total polysaccharide in garlic polysaccharide. Method: Glucose was used as a reference substance
and phenol-sulfuric acid was applied as coloring reagent to determine the content of total polysaccharide. Result: The calibration curve of glucose showed good linearity in the range of 0.011 6-0.057 8 g·L-1 (r=0.999 5). The average recovery rate was 101.4%
and RSD was 2.1%. Conclusion: The determination method is simple
sensitive and accurate
which can be used for evaluating the quality of total polysaccharide in the garlic polysaccharide.