Antioxidant Effects of Addition and Subtraction Bufei Decoction on Pulmonary Fibrosis in Rats
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Antioxidant Effects of Addition and Subtraction Bufei Decoction on Pulmonary Fibrosis in Rats
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 18, Pages: 236-239(2012)
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Published:2012
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XIE Gao-yu, YANG Bing-yao, OU Jiang-qin, et al. Antioxidant Effects of Addition and Subtraction Bufei Decoction on Pulmonary Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 236-239.
DOI:
XIE Gao-yu, YANG Bing-yao, OU Jiang-qin, et al. Antioxidant Effects of Addition and Subtraction Bufei Decoction on Pulmonary Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 236-239.DOI:
Antioxidant Effects of Addition and Subtraction Bufei Decoction on Pulmonary Fibrosis in Rats
Objective: To investigate the antioxidant effects of addition and subtraction Bufei decoction (ASBD) on pulmonary fibrosis (PF) in rats. Method: Forty male Sprague-Dawley rats were divided randomly into sham operation group
model group
ASBD group. The PF model was reproduced by intratracheal injection of bleomycin. ASBD group was given traditional Chinese medicine decoction (ig
2 times to clinic dose
16 g · kg-1) starting from 2 days after operation. The sham operation group and model group were given the same amount of normal saline. The blood and lung tissue of rats were took on the 14 d and 28 d. The superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in serum were measured. The content of hydroxyproline in lung tissue was detected. The mRNA of extracellular SOD(ECSOD) was measured by real-time PCR. Result: ASBD significantly improved the survival state of PF rats
reduced content of hydroxyproline in the lung tissue
decreased the level of MDA in the serum
improved the expression of ECSOD mRNA. Conclusions: ASBD could improve the survival rate of PF rats
and the fibrosis index. The mechanism might be related to decrease in the level of serum MDA and up-regulation of the expression of ECSOD mRNA.
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XIE Gao-yu1
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OU Jiang-qin3
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CHEN Yun-zhi1
WANG Jian-ke1
YANG Chang-fu
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