Effects of Qize Decoction on the Expression of Endoplasmic Reticulum Stress Related Apoptosis Molecules in Chronic Renal Failure Rats

WANG Sheng-fei; CHEN Li-guo; QU Yuan; YAN Liang; TANG Hai-lan; CHEN Ming-yi; WANG Hua-qiang

您当前的位置：

首页 >

文章列表页 >

Effects of Qize Decoction on the Expression of Endoplasmic Reticulum Stress Related Apoptosis Molecules in Chronic Renal Failure Rats

更新时间：2024-01-04

- Effects of Qize Decoction on the Expression of Endoplasmic Reticulum Stress Related Apoptosis Molecules in Chronic Renal Failure Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 11, Pages: 162-166(2012)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2012
- 稿件说明：
移动端阅览
WANG Sheng-fei, CHEN Li-guo, QU Yuan, et al. Effects of Qize Decoction on the Expression of Endoplasmic Reticulum Stress Related Apoptosis Molecules in Chronic Renal Failure Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 162-166.
DOI：

WANG Sheng-fei, CHEN Li-guo, QU Yuan, et al. Effects of Qize Decoction on the Expression of Endoplasmic Reticulum Stress Related Apoptosis Molecules in Chronic Renal Failure Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 162-166. DOI：

摘要

目的: 探讨芪泽汤对慢性肾功能衰竭(CRF)大鼠内质网应激(ERS)相关凋亡分子表达的影响。方法: 2.5%腺嘌呤ig24 d建立SD大鼠肾衰模型。分为5组:正常组、模型组、尿毒清组(2.5 g·kg-1·d-1)、芪泽汤高、低剂量组(50.4

12.6 g·kg-1·d-1)

每组10只。造模同时各治疗组ig给药

干预24 d后

观察体重变化

全自动生化分析仪检测大鼠血尿素氮(BUN)、血肌酐(SCr)

应用TUNEL法检测肾组织细胞凋亡

Western blot检测CHOP(C/EBP-homologous protein)和半胱氨酸天冬氨酸蛋白酶12(Caspase-12)肾内表达水平。结果: 模型组BUN(17.21 ±4.63)mmol·L-1

SCr(67.00±25.62)μmol·L-1较对照组BUN(4.19±0.77)mmol·L-1

SCr(15.89±2.62)μmol·L-1明显升高(P<0.01);TUNEL结果显示空白组肾小管上皮细胞有少量凋亡

而模型组细胞凋亡数明显增多(P<0.01);模型组CHOP

Caspase-12表达显著增强(P<0.01)。芪泽汤低剂量和尿毒清能抑制慢性肾功能衰竭大鼠肾小管上皮细胞凋亡和CHOP

Caspase-12表达(与模型组相比较P<0.05)。结论: 芪泽汤低剂量能保护腺嘌呤导致的大鼠肾损害

可能与其抑制ERS相关凋亡分子CHOP

Caspase-12高表达有关。

Abstract

Objective: To study the effect of Qize decoction at endoplasmic reticulum stress(ERS) in rats with chronic renal failure(CRF). Method: SD rats were fed with adenine of 2.5% to establish chronic renal failure model. They were divided into five groups:normal group

model group

Niaoduqing group(2.5 g·kg-1·d-1)

Qize decoction of high dosage(50.4 g·kg-1·d-1)group and Qize decoction of low dosage(12.6 g·kg-1·d-1) group

each group has 10 rats. All groups were infused with adenine into stomach in addition to normal group

at the same time

each rat in chinese herbal compound group was also infused with chinese herbal compound into stomach and Niaoduqing group was given Niaoduqing liquid for 24 days. Body weight was observed dynamically

blood urea nitrogen(BUN) and serum creatinine(SCr) levels were checked by automatic biochemical analyzer.The apoptosis of renal was evaluated by means of terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling(TUNEL).The expression of C/EBP-homologous protein(CHOP) and Caspase-12 was detected by Western bloting. Result: Compared with the control group BUN(4.19±0.77)mmol·L-1 and SCr(15.89±2.62)μmol·L-1

in the model group BUN(17.21 ±4.63) mmol·L-1 and SCr levels (67.00 ±25.62) μmol·L-1 were much higher(P<0.01).The results of TUNEL showed that the few numbers of apoptosis in renal tubular epithelial cells in normal group were found

but more in model group. The expression of CHOP and Caspase-12(P<0.01)were much higher in the model group. Compared with the model group

the number of apoptosis in renal tubular epithelial cells and the expression of CHOP and Caspase-12 were reduced(P<0.05) in Qize decoction low dosage group and Niaoduqing group. Conclusion: Qize decoction low dosage could protect the adenine-induced renal damage

maybe related to that it can inhibit the high expression of ERS-related apoptosis-molecule CHOP and Caspase-12.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Role of Endoplasmic Reticulum Stress in Atherosclerosis and Its Regulation by Traditional Chinese Medicine： A Review

Mechanism of Yishen Tongluo Prescription in Inhibiting Endoplasmic Reticulum Stress and Improving Apoptosis of Renal Tubular Epithelial Cells Based on PERK/ATF4/CHOP

Mechanism of Wenxin Granule Inhibits Myocardial Cell Apoptosis After Myocardial Infarctionby Regulating Endoplasmic Reticulum Stress in Rats

Modified Shuyuwan Ameliorate Neuronal Apoptosis of APP/PS1 Mice Via Akt/GSK3β/Nrf2 Pathway

Related Author

LIANG Qingzhi

CHEN Zhengtao

ZHOU Ruoran

LI Jiying

ZHANG Yuan

XIE Chunguang

YANG Qiyue

SU Xuan

Related Institution

Hospital of Chengdu University of Traditional Chinese Medicine （TCM）

Chengdu University of TCM

First Affiliated Hospital of Army Medical University

Academy of Chinese Medica Sciences， Henan University of Chinese Medicine

Dongzhimen Hospital，Ministry of Education and Beijing Key Laboratory of Traditional Chinese Medicine Internal Medicine， Beijing University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰