Effects of Huanglian Wendan Decoction on Experimental Autoimmune Neuritis in Lewis Rats

BEN Ying; WANG Xiu-li; ZHANG Feng-hua; LIANG Wen-jie; ZHANG Pei-nan

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Effects of Huanglian Wendan Decoction on Experimental Autoimmune Neuritis in Lewis Rats

更新时间：2024-09-23

- Effects of Huanglian Wendan Decoction on Experimental Autoimmune Neuritis in Lewis Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 9, Pages: 237-242(2012)
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- Published：2012
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BEN Ying, WANG Xiu-li, ZHANG Feng-hua, et al. Effects of Huanglian Wendan Decoction on Experimental Autoimmune Neuritis in Lewis Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 237-242.
DOI：

BEN Ying, WANG Xiu-li, ZHANG Feng-hua, et al. Effects of Huanglian Wendan Decoction on Experimental Autoimmune Neuritis in Lewis Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 237-242. DOI：

摘要

目的: 探讨黄连温胆汤加减方对大鼠实验性自身免疫性神经炎(EAN)的影响及其机制。方法: 应用周围神经髓鞘抗原(P257-81多肽)与完全弗氏佐剂的混合液免疫Lewis大鼠

建立EAN动物模型并随机分为黄连温胆汤加减方高剂量组(HL高组)、黄连温胆汤加减方低剂量组(HL低组)、EAN模型组、正常组。HL高组和HL低组于免疫当天至第15天每天固定时段灌胃给予黄连温胆汤加减方浓煎剂1次

剂量分别为26.89

6.79 g·kg-1·d-1。观察各组发病情况

坐骨神经中T细胞、巨噬细胞浸润及组织病理学变化

检测外周血中17型辅助T细胞(Th17细胞)和叉头状转录因子3阳性调节T细胞(Foxp3+Treg细胞)比例和淋巴结中肿瘤坏死因子-α(TNF-α)、干扰素-γ(IFN-γ)、白细胞介素-6(IL-6)、白细胞介素-17(IL-17)mRNA水平。结果: HL高组的最初发病时间为(11.92±1.38)d

迟于EAN组(P＜0.05)

其高峰期临床评分为(2.41±1.09)

髓鞘脱失的组织学评分为(0.58±0.51)

均显著低于EAN组(P＜0.05);HL高组和HL低组T细胞、巨噬细胞浸润明显减少

外周血中Th17细胞比例(1.88±0.47)%

(2.71±0.39)%较EAN组显著减少(P＜0.05)

Foxp3+Treg细胞比例(15.06±1.35)%

(9.18±1.00)%较EAN组显著增加(P＜0.05)

淋巴结中促炎细胞因子TNF-α

IFN-γ

IL-6

IL-17mRNA表达HL高组分别为(0.38±0.02)%

(0.45±0.08)%

(0.34±0.13)%

(0.63±0.17)%;HL低组分别为(0.45±0.03)%

(0.60±0.05)%

(0.74±0.12)%

(1.04±0.17)%与EAN组比较明显下降(P＜0.05)。结论: 黄连温胆汤加减方能够抑制EAN大鼠的自身免疫反应

对EAN有治疗作用。

Abstract

Objective: To explore the effects and mechanism of Huanglian Wendan decoction on experimental autoimmune neuritis (EAN). Method: EAN model was established by immunizing Lewis rats with peripheral nerve myelin sheath antigen (P257-81)peptide and complete Freund's adjuvant (CFA). Lewis rats were randomly divided into Huanglian Wendan decoction high-dose group and low-dose group

the EAN model group and normal group. Huanglian Wendan decoction high-dose group and low-dose group were lavaged with 26.89 g·kg-1·d-1 or 6.79 g·kg-1·d-1 once daily from day 0 to day 15 post-immunization. The effects were assessed in terms of appearance of clinical signs

histopathology and T cells

macrophages infiltrating in sciatic nerve sections

T help 17 (Th17) cells and Foxp3+ regulatory T (Foxp3+Treg) cells in peripheral blood

mRNA levels of inflammatory cytokines: tumor necrosis factor-α (TNF-α)

interferon-γ (IFN-γ)

interleukin-6 (IL-6) and interleukin-17(IL-17) in the lymph nodes. Result: Huanglian Wendan decoction high-dose group primaryneurological signs were found at day 11.92±1.38

it was later than that in EAN group. The maximal neurological score (2.41±1.09) and the mean histological scores (0.58±0.51) of high-dose group were lower than that in EAN group. Huanglian Wendan decoction high-dose group and low-dose group reduced accumulation of macrophages and T cells in sciatic nerves. In peripheral blood

Foxp3+Treg cells in high-dose group and low-dose group (15.06±1.35

9.18±1.00)% were increased

but Th17 cells (1.88±0.47

2.71±0.39)%were decreased. Furthermore

TNF-α

IFN-γ

IL-6

IL-17mRNA levels in the lymph nodes of high-dose group (0.38±0.02)%

(0.45±0.08)%

(0.34±0.13)%

(0.63±0.17)%and low-dose group (0.45±0.03)%

(0.60±0.05)%

(0.74±0.12)%

(1.04±0.17)% significantly suppressed. Conclusion: Huanglian Wendan decoction could effectively suppress autoimmune response in EAN

it can treat EAN effectively.

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Related Author

BEN Ying1

WANG Xiu-li2

ZHANG Feng-hua1

LIANG Wen-jie1

ZHANG Pei-nan1

SHEN Zhixiang

LU Weimin

SHI Chuan

Related Institution

The Second Affiliated Hospital of Hebei Medical University

Integrated Chinese Traditional and Western Medical College of Hebei Medical University

Affiliated Hospital of Nanjing University of Chinese Medicine

The Second Affiliated Hospital of Shandong University of TCM

The First Clinical Medical College，Shandong University of Traditional Chinese Medicine（TCM）

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