Main Active Section on Human Umbilical Vein Endothelial Cells Hypoxia/reoxygenation Injury Model in Shengmai Injection
|更新时间:2024-01-04
|
Main Active Section on Human Umbilical Vein Endothelial Cells Hypoxia/reoxygenation Injury Model in Shengmai Injection
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 13, Pages: 88-90(2012)
作者机构:
作者简介:
基金信息:
DOI:
CLC:
Published:2012
稿件说明:
移动端阅览
JIANG Sheng-lan, ZHU Dan-ni, YU Bo-yang. Main Active Section on Human Umbilical Vein Endothelial Cells Hypoxia/reoxygenation Injury Model in Shengmai Injection[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 88-90.
DOI:
JIANG Sheng-lan, ZHU Dan-ni, YU Bo-yang. Main Active Section on Human Umbilical Vein Endothelial Cells Hypoxia/reoxygenation Injury Model in Shengmai Injection[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 88-90.DOI:
Main Active Section on Human Umbilical Vein Endothelial Cells Hypoxia/reoxygenation Injury Model in Shengmai Injection
Objective: To study the material basis of Shengmai injection by hypoxia/reoxygenation (H/R) injury. Method: Shengmai injection was separated into three fractions according to semi-preparative HPLC retention time to investigate their protective effects on human umbilical vein endothelial cells(HUVEC)H/R injury model
respectively. Result: 50-80 min elute was proved to be the main active section of Shengmai injection on HUVEC H/R injury model with the highest protection rate of 52.76%
HPLC-MS analysis indicated that ginsenosides were the main active component in this fraction. Conclusion: The medium-polar fraction containing ginsenosides showed most remarkable protective effects on HUVEC H/R injury in Shengmai injection.