Experimental Study on Protective Effects of Pabing Ⅱ Formula in Parkinson's Disease Rats

WEN Xiao-dong; LUO Xiao-dong; WANG Chun-ling

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Experimental Study on Protective Effects of Pabing Ⅱ Formula in Parkinson's Disease Rats

更新时间：2024-09-23

- Experimental Study on Protective Effects of Pabing Ⅱ Formula in Parkinson's Disease Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 9, Pages: 224-228(2012)
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- Published：2012
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WEN Xiao-dong, LUO Xiao-dong, WANG Chun-ling. Experimental Study on Protective Effects of Pabing Ⅱ Formula in Parkinson's Disease Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 224-228.
DOI：

WEN Xiao-dong, LUO Xiao-dong, WANG Chun-ling. Experimental Study on Protective Effects of Pabing Ⅱ Formula in Parkinson's Disease Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(9): 224-228. DOI：

摘要

目的:研究帕病2号方对帕金森病(PD)模型大鼠的抗氧化作用。方法:采用6-羟基多巴胺(6-OHDA)纹状体两点注射法造成左侧纹状体损毁模型

ip阿朴吗啡(APO)诱导并诊断大鼠旋转行为

将造模成功大鼠随机分为帕病2号方高、中、低剂量组(32.0

16.0

8.0 g·kg-1)、美多巴组(0.075 g·kg-1)、模型组

连续ig 4周

同时设立正常组

正常组与模型对照组给予等容积蒸馏水;应用比色法测定大鼠纹状体匀浆中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性及丙二醛(MDA)的含量

ip APO诊断大鼠旋转行为的变化。结果:神经行为学方面

模型组大鼠治疗前后旋转圈数无显著差异

帕病2号方高、中剂量组大鼠旋转圈数较模型组显著减少(P<0.05)

且治疗前后比较差异显著 (P<0.05);美多巴组大鼠旋转圈数亦明显减少(P<0.05)。模型组MDA(13.48±3.71) nmol·mg-1明显升高

GSH-Px

SOD(497.48±72.61) NU·g-1

(113.11±8.08) U·mg-1 明显降低

与正常对照组相比差异显著(P<0.05或 P<0.01);帕病2号方高、中剂量组可明显降低MDA(9.29±3.25)

(9.62±2.78)nmol·mg-1

提高GSH-Px

SOD活性[分别为(612.53±53.20)

(637.03±133.21) NU·g-1及(123.93±9.84)

(120.21±10.63) U·mg-1](P<0.05);低剂量组仅GSH-Px增加(573.19±54.86 )NU·g-1

MDA

SOD则无显著改变。美多巴组上述指标均无显著改善。结论: 帕病2号方明显改善PD模型大鼠的旋转行为

提高其抗氧化能力和清除自由基的能力

并呈明显的量效关系。

Abstract

Objective:To observe the effect of the antioxidation on Parbin II formula in Parkinson's disease (PD) rats. Method: PD rats were induced by injection of 6-hydroxydopamine (6-OHDA) twice stereotaxically into the left side of striatum

the rotation test was diagnosed by injecting apomorphin (APO)

the PD rats were randomly divided into five groups: model group

Parbin Ⅱ formula (32.0

16.0

8.0 g·kg-1) and madopar group(0.075 g·kg-1)

at the same time

the normal group was established and the treatment lasted for 4 weeks. The model group and the normal group were given vehicle only. The colorimetric assays were used to detect the activities of glutathione peroxidase(GSH-Px)

superoxidase dismutase (SOD) and the levels of malonaldehyde (MDA) in tissue homogenate that came from the the left side of striatum and behavioural changes were detected by injection of APO after the treatment. Result: There was no significant difference in the rotation behavior between before and after treatment in model group; compared with model group

the rotation behavior was significantly decreased in Parbin Ⅱ formula high and media dose group(P<0.05)

and there was a significant difference between before and after treatment (P<0.05). Madopar could also change the rotation behavior in PD rats. However

Parbin Ⅱ formula low dose group had no change. Compared with the normal control group

the contents of MDA were obviously increased and the activities of GSH-Px and SOD were obviously reduced in model group (P<0.05). However

the above indicators were improved in Parbin Ⅱ Formula treatment group

the high

media dose group can dereased obviously MDA contents and had the significant difference (P<0.05)

which were (9.29±3.25)

(9.62±2.78) nmol·mg-1 respectively

increased obviously the activities of GSH-Px and SOD (P<0.05)

which was (612.53±53.20)

(637.03±133.21) NU·g-1

(123.93±9.84)

(120.21±10.63) U·mg-1 respectively; low dose group could only remove the activities of GSH-Px and had no change in MDA and SOD contents. However

there was no significant difference in the above indicators in madopar group. Conclusions: Pabing Ⅱ formula can significantly improve the rotation behavior of PD model rats

enhance antioxidation ability and eliminate free radicals ability

and its effect is in a dose dependent manner.

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Related Author

WEN Xiao-dong1

LUO Xiao-dong2

WANG Chun-ling3

ZHENG Qi

LU Yi

YU Donghua

ZHAO Liangyou

LIN Chunsheng

Related Institution

Guangzhou University of Traditional Chinese Medicine

Guangdong Province Hospital of Traditional Chinese Medicine

Guangxi Traditional Chinese Medical University

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