Optimization of Extration Technology for Astragaloside Ⅳ from by Response Surface Methodology
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Optimization of Extration Technology for Astragaloside Ⅳ from by Response Surface Methodology
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 18, Pages: 34-37(2012)
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Published:2012
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SHEN Xiao-zhong, YANG Fan, YU Rong-min. Optimization of Extration Technology for Astragaloside Ⅳ from by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 34-37.
DOI:
SHEN Xiao-zhong, YANG Fan, YU Rong-min. Optimization of Extration Technology for Astragaloside Ⅳ from by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 34-37.DOI:
Optimization of Extration Technology for Astragaloside Ⅳ from by Response Surface Methodology
Objective: To optimize extraction technology of astragaloside Ⅳ from Astragalus membranaceus by response surface methodology(RSM). Method: Three-factor and three-level Box-Behnken experimental design was employed with ethanol concentration
flow rate and liquid-solid ratioas independence variables
extraction rate of astragaloside Ⅳ as dependent variable which was determined by HPLC-ELSD
experimental data obtained by multiple regression and binomial fitting of levels from independent variables
ethanol percocation extraction technology was optimized by RSM
and to predictive analysis. Result: Optimum extraction conditions were as follows:30% ethanol as solvent
flow rate 1.9 mL · min-1 · cm-2
liquid-solid ratio 6.5 mL · g-1.Under these conditions
theoretical value of extraction rate of astragaloside Ⅳ 1.50 mg · g-1
but measured value of 1.487 mg · g-1
it showed optimized technology model fitted well. Conclusion: Box-Behnken experimental design could be used for extraction optimization of percocation extraction process for A. membranaceus
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Related Author
SHEN Xiao-zhong1
YANG Fan3
YU Rong-min2
LI Guoting
YANG Changchao
LIU Lin
LI Weikang
ZHAO Zixian
Related Institution
Chongqing Institute for Food and Drug Control
College of Pharmacy,Ji’nan University
Guangdong Food and Drug Vocational College
Hebei Key Laboratory of Traditional Chinese Medicine(TCM) Research on Cardiocerebrovascular Disease,TCM Processing Technology Innovation Center of Hebei Province,College of Pharmacy, Hebei University of Chinese Medicine
Key Laboratory of Xin'an Medical Education Department,Anhui University of Chinese Medicine