Objective: To evaluate the anti-tumor effects of neogambogic acid on S180 cell line both in vivo and in vitro. Method: CCK-8 method was used to assay the anti-proliferative effect of neogambogic acid with different concentrations for 48 h on S180 cell line in vitro. The anti-tumor effect in vivo was evaluated by the survival time of the mice bearing S180 ascitic tumor

which was ip treated with 8.0

4.0

2.0 mg·kg-1 neogambogic acid respectively

once per day for continuous 7 days

and observation period lasted for 45 days

and the mice bearing S180 xenograft was ig treated with 16.0

8.0

4.0 mg·kg-1 neogambogic acid respectively

once per day for continuous 12 days. The LD50 of mice was determined based on single administration (ip)of different doses of neogambogic acid. Result: Neogambogic acid showed obvious anti-proliferative effect on S180 cell culture

the IC50 of 48 h was (1.54±0.12) mg·L-1. And the in vivo test showed that the survival time of the mice administrated with 4.0 mg·kg-1 neogambogic acid was prolonged 141.6%. The inhibitory effects of the mice bearing tumor increased with the increasing of the concentration of neogambogic acid in a dose-dependent manner (P<0.05). The LD50 of neogambogic acid (ip) was 36.66 mg·kg-1. Conclusion: The results suggest that the anti-tumor effect of neogambogic acid on S180 cell line is significant both in vivo and in vitro.