Optimization of Purification Process for Total Flavonoids from Leaves of by Macroporous Resin

LIU Yun; ZHU Xin-ting

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Optimization of Purification Process for Total Flavonoids from Leaves of by Macroporous Resin

更新时间：2024-01-04

- Optimization of Purification Process for Total Flavonoids from Leaves of by Macroporous Resin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 11, Pages: 55-57(2012)
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- Published：2012
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LIU Yun, ZHU Xin-ting. Optimization of Purification Process for Total Flavonoids from Leaves of by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 55-57.
DOI：

LIU Yun, ZHU Xin-ting. Optimization of Purification Process for Total Flavonoids from Leaves of by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 55-57. DOI：

摘要

目的: 优选大孔树脂吸附法富集枇杷叶总黄酮的工艺条件。方法: 以芦丁为对照品

硝酸铝显色法检测枇杷叶总黄酮的含量。以静态吸附容量、静态解吸率为考察指标

比较6 种大孔树脂对枇杷叶总黄酮的吸附和解吸效果

筛选最佳大孔树脂型号

并对其吸附和解吸条件进行探讨。结果: HPD100型大孔树脂最适合于枇杷叶总黄酮的纯化

其纯化工艺为上样液质量浓度3 g·L-1

上样速率2 BV·h-1

上样液体积2.5 BV

2 BV去离子水冲洗

5 BV 70%乙醇以1 BV·h-1流速洗脱

收集洗脱液。在此工艺条件下

总黄酮得率78.7%

总黄酮纯度47.3%。结论: HPD100型大孔树脂对枇杷叶总黄酮具有良好的纯化效果

优选工艺合理、稳定可行。

Abstract

Objective: To optimize enrichment technology conditions of total flavonoids from leaves of Eriobotrya japonica by macroporous adsorption resin. Method: With rutin as reference substance

the content of total flavonoids was determined by aluminium nitrate chromogenic method.With static adsorption capacity and static desorption rate as indexes

optimum type of macroporous resin was selected by comparing adsorption and desorption effect of 6 kinds macroporous resin for total flavonoids from leaves of E. japonica

and to investigate its absorption and desorption conditions. Result: HPD 100 macroporous resin was the most suitable for purification of total flavonoids from E. japonica.Optimum purification conditions were:the concentration of sampling liquid of 3 g·L-1

sampling velocity 2 BV·h-1

sample liquid volume 2.5 BV

eluted by 2 BV deionized water and 5 BV 70% ethanol at the speed of 1 BV·h-1

eluate was collected.Under these process conditions

yield of total flavonoids was 78.7%

purity of total flavonoids was 47.3%. Conclusion: HPD 100 macroporous resin had a good purification effect of total flavonoids from leaves of E. japonica

optimized purification process was reasonable

stable and feasible.

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Related Institution

Affiliated Hospital of Jiangsu University

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Southen University Key Lab of Traditional Chinese Medicine and New Drug,Guangdong Provincial Key Lab of Traditional Chinese Medicine

Zhejiang Chinese Medical University

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