Determination of the Concentration of Crocin-Ⅰ and Crocin-Ⅱ in jasminoides by HPLC
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Determination of the Concentration of Crocin-Ⅰ and Crocin-Ⅱ in jasminoides by HPLC
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 17, Pages: 68-70(2012)
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Published:2012
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GAO Feng-yang, GAO Xiao-yan, LU Jian-qiu, et al. Determination of the Concentration of Crocin-Ⅰ and Crocin-Ⅱ in jasminoides by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(17): 68-70.
DOI:
GAO Feng-yang, GAO Xiao-yan, LU Jian-qiu, et al. Determination of the Concentration of Crocin-Ⅰ and Crocin-Ⅱ in jasminoides by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(17): 68-70.DOI:
Determination of the Concentration of Crocin-Ⅰ and Crocin-Ⅱ in jasminoides by HPLC
Objective: To establish the method for determination of crocin-Ⅰand crocin-Ⅱ in the Gardenia jasminoides. Method: High performance liquid chromatography was applied on a Dikma Diamonsil C18 (4.6 mm × 250 mm
5 μm) column at temperature 30 ℃ with 0.1% formic acid-acetonitrile as the mobile phase in gradient elution for 30 min. The flow rate was 1.0 mL·min-1
detected wavelength of 440 nm. Result: The sample quality of crocin-Ⅰand crocin-Ⅱwith the peak area showed good linear relationship; the average recoveries were 96.68% (n=6) and 102.94% (n=6). Conclusion: The method is simple
accurate and reproducible; it can provide the basis of rational application
quality control and the standards for G. jasminoides.