Determination of Kaempfero 3, 7----dirhamnopyranoside in  from Different Habitats by HPLC

MA Bing-zhi; JU Hai; TIAN Xue-feng; WANG Wei; ZHU Li-ping; GAO Zeng-ping

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Determination of Kaempfero 3, 7----dirhamnopyranoside in from Different Habitats by HPLC

更新时间：2024-01-04

- Determination of Kaempfero 3, 7----dirhamnopyranoside in from Different Habitats by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 12, Pages: 130-131(2012)
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- Published：2012
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MA Bing-zhi, JU Hai, TIAN Xue-feng, et al. Determination of Kaempfero 3, 7----dirhamnopyranoside in from Different Habitats by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(12): 130-131.
DOI：

MA Bing-zhi, JU Hai, TIAN Xue-feng, et al. Determination of Kaempfero 3, 7----dirhamnopyranoside in from Different Habitats by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(12): 130-131. DOI：

摘要

目的: 建立高效液相色谱法测定单芽狗脊中山柰酚3

7-O-α-L-二鼠李糖苷含量的方法。方法: HPLC条件为DiamonsilTMC18 (4.6 mm×200 mm

5 μm) 色谱柱

流动相乙腈-水(30∶70)

检测波长265 nm

流速1.0 mL·min-1

柱温25 ℃。结果: 9个产地单芽狗脊中山柰酚3

7-O-α-L-二鼠李糖苷的质量分数范围为0.05%~0.43%。结论: 该法简便、准确、专属性强

可用于单芽狗脊中山奈酚-3

7-O-α-L-二鼠李糖苷的含量测定。

Abstract

Objective: To develop a HPLC method for the content determination of kaempferol 3

7-O-α-L-dirhamnopyranoside in Woodwardiae unigemmata. Method: HPLC was applied with a DiamonsilTMC18 column by acetonitrile-water (30∶70) as mobile phase. The flow rate was 1.0 mL·min-1. The column temperature was at 25 ℃ and the UV detection wavelength was at 265 nm. Result: The content range of kaempferol 3

7-O-α-L-dirhamnopyranoside from 9 different habats was from 0.05%-0.43%. Conclusion: The method is simple

accurate and specific. It can be used for determination of kaempferol 3

7-O-α-L-dirhamnopyranoside in W. unigemmata.

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Related Author

Hong-xing MA

Long-fei LIN

Yu-ling LIU

Sai FU

Jin-xin SHAO

Ji-zhong ZHU

Chuan-gui LIU

Hui LI

Related Institution

Jilin Agricultural University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Chengde Medical University

Jilin Huakang Pharmaceutical Co. Ltd.

The First Clinical Medical College of Guangzhou University of Chinese Medicine

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