Objective: To compare the total alkaloids and three kinds of diester diterpenoid alkaloids in different parts of monkshood medicinal material. Method: We used acid dye colorimetry method to evaluate the alkaloids and RP-HPLC to evaluate three kinds of diester diterpenoid alkaloids. The compounds was analyzed by gradient elution program performed with acetonitrile and 40 mmol·L-1 ammonium acetate as a mobile phase. The flow rate was 1.0 mL·min-1.The detection was done at 240 nm and column temperature was 30℃. Result: The total alkaloid content of different tissues which be harvested before June 4

was significantly less than on June 18 and July 3. The best harvest period of aconitine can be defined as the end of June and early July. The alkaloid content of ground parts of Aconitum is less than the underground. The contents of alkaloid and three kinds of diester diterpenoid alkaloids in different tissues of Aconitum carmichaeli were in the sequence of: fibrous tubers >monkshood-tubers > mother tubers > leaves > stem. Conclusion: The study was a preliminary understanding of the dynamic variation of the various tissues of the Aconitum Alkaloids and From the point of view on the content of alkaloids

the best harvest period of aconitine and content of alkaloids in various tissues can be defined.