Influence of Qiju Dihuang Decoction on Caspase-3 Expression in Retinal Degeneration Rats

CHEN Jin-mao; YANG Jin-nan; LIN Shao-chun; WU Kai-li

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Influence of Qiju Dihuang Decoction on Caspase-3 Expression in Retinal Degeneration Rats

更新时间：2024-01-04

- Influence of Qiju Dihuang Decoction on Caspase-3 Expression in Retinal Degeneration Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 11, Pages: 146-150(2012)
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- Published：2012
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CHEN Jin-mao, YANG Jin-nan, LIN Shao-chun, et al. Influence of Qiju Dihuang Decoction on Caspase-3 Expression in Retinal Degeneration Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 146-150.
DOI：

CHEN Jin-mao, YANG Jin-nan, LIN Shao-chun, et al. Influence of Qiju Dihuang Decoction on Caspase-3 Expression in Retinal Degeneration Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 146-150. DOI：

摘要

目的: 观察杞菊地黄汤对实验性视网膜变性大鼠的治疗机制。方法: 将生后45 d的SD大鼠分为正常组、模型对照组和杞菊地黄汤组

正常组不做处理

生后47 d中药组大鼠灌服杞菊地黄汤8.3 g·kg-1(15 mL·kg-1)

模型对照组同时灌服等体积生理盐水

生后50 d ip N-甲基-N-亚硝脲(N-methyl-N-nitrosourea

MNU)40 mg·kg-1造成视网膜变性模型

正常组第55天处死

后2组大鼠分别按造模后12 h

5 d处死

应用免疫组织化学法检测视网膜中半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)的表达

实时荧光定量RT-PCR法检测视网膜中Caspase-3的含量。结果: 免疫组织化学和实时荧光定量RT-PCR法均显示模型对照组大鼠视网膜Caspase-3阳性表达在MNU处理后渐升高

第2天达顶峰

第5天有所下降;杞菊地黄汤组大鼠视网膜Caspase-3阳性表达第3天才达顶峰

且高峰值低于模型组。结论: 杞菊地黄汤能延缓MNU ip所导致的大鼠视功能损害

其治疗机制与降低视网膜中Caspase-3的表达

从而抑制光感受器细胞的凋亡有关。

Abstract

Objective: To explore the influence of Qiju Dihuang decoction on Caspase-3 expression in N-methyl-N-nitrosourea (MNU)-induced retinal degeneration rats. Method: Sprague Dawley rats were divided into three groups: the control group

the model group and the Qiju Dihuang decoction group. MNU at dose 40 mg·kg-1 was ip injected at 50 d after birth to induce retinal degeneration model. All rats were sacrificed at scheduled time points.The expression of Caspase-3 on the retina was detected by immuohistochemistry and Real-Time PCR. Result: The results were demonstrated by immuohistochemistry and Real-Time PCR. Positive Caspase-3 expression in the model group was detected at 12 h after ip MNU

reached to the peak at 2 d and gradually reduced

whereas Caspase-3 expression in the Qiju Dihuang decoction group reached to the peak at 3 d and the peak value was lower than the former. Conclusion: The positive expression of Caspase-3 was raised after MNU injection. Qiju Dihuang decoction can restrain the apoptosis of photoreceptor cells by decreasing the expression of Caspase-3.

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Related Author

CHEN Jin-mao1

YANG Jin-nan2

LIN Shao-chun3

WU Kai-li3

PAN Shu-yu

CHEN Xiao-feng

XIAO Jing

PAN Hai-qiang

Related Institution

The First Affiliated Hospital,Guangxi Medical University

Xinxiang Medical College

Zhongshan Ophthalmic Center,Sun Yat-sen University

Affiliated Hospital of Nanjing University of Chinese Medicine

The First Clinical Medical College，Nanjing University of Chinese Medicine

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