UPLC Determination of Lactone in  Preparation

HUANG Peng-na

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UPLC Determination of Lactone in Preparation

更新时间：2024-01-04

- UPLC Determination of Lactone in Preparation
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 14, Pages: 98-101(2012)
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- Published：2012
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HUANG Peng-na. UPLC Determination of Lactone in Preparation[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(14): 98-101.
DOI：

HUANG Peng-na. UPLC Determination of Lactone in Preparation[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(14): 98-101. DOI：

摘要

目的: 建立UPLC-PDA技术快速测定穿心莲药材及制剂中主要内酯类成分含量的方法。方法: 采用超高效液相色谱仪

用Acquity BEH Shield RP C18色谱柱(2.1 mm×50 mm

1.7 μm);流动相甲醇(A)-水(B)

梯度洗脱(0~2 min

40%A;2~4 min

40%~45%A;4~10 min

45%~50% A;10~15 min

50% A);流速0.5 mL·min-1

检测波长226 nm

柱温30 ℃。结果: 在15 min内可以完成药材和制剂中穿心莲内酯、脱水穿心莲内酯的分析

分离度良好

线性范围分别为0.028 6~0.286 g·L-1(r＝0.999 6

n=6)

0.094 0~0.940 g·L-1(r＝0.999 3

n=6);平均加样回收率分别为97.32％

97.11％。结论: 该方法快速、准确、灵敏度高

可用于穿心莲药材及制剂的质量控制。

Abstract

Objective: To develop an UPLC method for the Determination of lactone in Andrographis paniculata peparation. Method: Acquity BEH Shield RP C18 column(2.1 mm×50 mm

1.7 μm) was used with Methanol(A)-Water(B) as a mobile phase by gradient elution program (0-2 min

40%A;2-4 min

40%-45% A;4-10 min

45%-50% A;10-15 min

50% A). The flow rate was 0.5 mL·min-1. The detecting wavelength was 226 nm. The column temperature was 30 ℃. Result: The linear ranges of andrographolide and dehydroandrographoline were 0.028 6-0.286 g·L-1(r＝0.999 6

n=6)

0.094 0-0.940 g·L-1(r＝0.999 3

n=6). The average recoveries were 97.32％ and 97.11％. Conclusion: The method was accurate

quickly and sensitive. It can be used for quality control of A.paniculata peparation.

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