Study on the Quality Control of Eucommiae Cortex by Multi-components Quantitation by One Marker Method and Fingerprint
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Study on the Quality Control of Eucommiae Cortex by Multi-components Quantitation by One Marker Method and Fingerprint
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 13, Pages: 78-82(2012)
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Published:2012
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LIN Fang, WANG Yun-hong, WAN Li, et al. Study on the Quality Control of Eucommiae Cortex by Multi-components Quantitation by One Marker Method and Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 78-82.
DOI:
LIN Fang, WANG Yun-hong, WAN Li, et al. Study on the Quality Control of Eucommiae Cortex by Multi-components Quantitation by One Marker Method and Fingerprint[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 78-82.DOI:
Study on the Quality Control of Eucommiae Cortex by Multi-components Quantitation by One Marker Method and Fingerprint
Objective: To establish the multi-indicator HPLC fingerprint of Eucommiae Cortex and to determine the contents of geniposidic acid
chlorogenic and acid
pinoresinol diglucoside with quantitative analysis of multi-components by single marker. Method: WelchromTM C18 110A (4.6 mm×250 mm
5 μm) column was used
the mobile phase was consisted of methanol-water-0.3% acetic acid as gradient elution. The column temperature was at 20℃.The flow rate was 1.0 mL·min-1 and the fingerprint was detected at 238 nm.Geniposidic acid and chlorogenic acid
pinoresinol diglucoside and geniposide was choosen as marks of ingredients to establish HPLC fingerprint. Using chlorogenic acid as internal reference standard
the contents of geniposidic acid
chlorogenic acid
pinoresinol diglucoside was determined according to the relative correction factor. Result: All tested samples contained the 12 common peaks and there was no significant differences between the quantitative results of three ingredients of quantitative analysis of multi-components by single marker method and external standard method. Conclusion: The multi-indicator HPLC fingerprint and simultaneous determination of geniposidic acid and chlorogenic acid
pinoresinol diglucoside with quantitative analysis of multi-components by single marker were firstly studied.This method was simple and feasible
which would provide better technique support for the quality control of Eucommiae Cortex.
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