Objective: To investigate the chemical constituents of the whole grass of Lespedeza virgat. Method: Various chromatographic techniques were used to separate and purify the chemical constituents from ethyl acetate part and n-butyl alcohol part extract of Lespedeza virgate

and the isolated compounds were identified according to their physicochemical and spectroscopic data. Result: Seven compounds were isolated and identified as physcion (1)

chrysophanol (2)

kaempferol-3

7-di-O-β-D-glucopy-ranoside (3)

kaempferol-3-O-β-D-rutinoside (4)

kaempferol-7-O-α-L-rhamnopyranoside (5)

n-hexacosanoic acid (6)

n-octacosanol (7). Conclusion: The compounds 1

2

3

4

6 were isolated for the first time from this genus

and the compound 7 was isolated from the plant for the first time.