Simultaneous Determination of Five Components in Tangminlingwan by HPLC

LEI Ding-chao; E Xiu-hui; ZHANG Lan-lan; ZHOU Shui-ping; YAN Xi-jun

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Simultaneous Determination of Five Components in Tangminlingwan by HPLC

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- Simultaneous Determination of Five Components in Tangminlingwan by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 13, Pages: 70-73(2012)
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- Published：2012
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LEI Ding-chao, E Xiu-hui, ZHANG Lan-lan, et al. Simultaneous Determination of Five Components in Tangminlingwan by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 70-73.
DOI：

LEI Ding-chao, E Xiu-hui, ZHANG Lan-lan, et al. Simultaneous Determination of Five Components in Tangminlingwan by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(13): 70-73. DOI：

摘要

目的: 建立HPLC同时测定糖敏灵丸中芍药内酯苷、芍药苷、柚皮苷、新橙皮苷及黄芩苷含量的方法。方法: 采用Aglient ZorbaxSB-C18色谱柱(4.6 mm×250 mm

5 μm)

以0.05%磷酸水溶液-乙腈为流动相进行梯度洗脱

流速1 mL·min-1

检测波长230 nm

柱温30℃。结果: 芍药内酯苷、芍药苷、柚皮苷、新橙皮苷及黄芩苷的线性范围分别为0.008~0.320

0.021~0.832

0.088~3.52

0.124~4.96

0.086~3.44 μg(r≥0.9999

n=7);平均回收率(n=6)分别为99.7%(RSD 2.0%)

100.1%(RSD 1.8%)

100.1%(RSD 1.5%)

100.0% (RSD 2.4%)

103.6%(RSD 2.4%)。结论: 所建立的HPLC方法简便

结果准确、可靠

重复性好。

Abstract

Objective: To establish an HPLC method for simultaneous detemination of albiflorin

paeoniflorin

naringin

neohesperidin and baicalin in Tangminlingwan. Method: The chromatographic method was carried out on an Aglient Zorbax SB-C18 column(4.6 mm×250 mm

5 μm) with acetonitrile-0.05% phosphoric acid solution as the mobile phase by gradient elution. The flow rate was 1 mL·min-1.The detection was set at the wavelength of 230 nm. The column temperature was at 30℃. Result: The linear ranges of albiflorin

paeoniflorin

naringin

neohesperidin and baicalin were 0.008-0.320

0.021-0.832

0.088-3.52

0.124-4.96 and 0.086-3.44 μg(r≥0.9999

n=7)

respectively. The average recoeries were 99.7%(RSD 2.0%)

100.1%(RSD 1.8%)

100.1%(RSD 1.5%)

100.0%(RSD 2.4%) and 103.6%(RSD 2.4%)

respectively. Conclusion: The method is simple

accurate and reproducible.

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