Determination of Luteolin in by HPLC

PAN Li; LIAO Hou-zhi

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Determination of Luteolin in by HPLC

更新时间：2024-01-04

- Determination of Luteolin in by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 17, Pages: 145-147(2012)
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- Published：2012
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PAN Li, LIAO Hou-zhi. Determination of Luteolin in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(17): 145-147.
DOI：

PAN Li, LIAO Hou-zhi. Determination of Luteolin in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(17): 145-147. DOI：

摘要

目的: 建立舞草中木犀草素的含量测定方法。方法: 采用VP-ODS C18(4.6 mm×250 mm

5 μm)色谱柱。以甲醇-0.3%磷酸溶液(60∶40)为流动相

流速1.0 mL·min-1

检测波长350 nm。结果: 舞草中的木犀草素含量测定方法在0.0407 2~0.6515 2进样量与峰面积的线性关系良好(r=0.999 8);平均回收率100.35%

RSD 0.80%。结论: 本方法准确、灵敏、重复性好

可用于舞草的质量控制。

Abstract

Objective: To establish the determination method of luteolin content in Codariocalyx motorius. Method: VP-ODS Cl8(4.6 mm×250 mm

5 μm) colmnn was used

the mobile phase was methanol-0.3% phosphonic acid solution (60∶40) with the flow rate as 1.0 mL·min-1. The detection wavelength was set at 350 nm. Result: The linear relation of hyperin was good in the range from 0.040 72 to 0.651 52 μg. The average recovery was 100.35%

RSD was 0.80%. Conclusion: The method established is accurate

sensitive and good in reproducibility. It can be applied to the quality control of C. motorius.

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