Objective: The establishment of infrared spectral identification for Cichorium glandulosum and C. intybus was carried out. Method: The use of infrared spectroscopy of three identification methods for C. glandulosum and C. intybus were analyzed and identified. Result: Infrared spectra showed that sugar and aromatic component content in the C. glandulosum was higher than C. intybus. Observing their secondary derivative spectra in the range of 1 800-1 300 cm- 1

the strongest peak of C. glandulosum is at 1 593 cm-1 and it is from the aromatic compounds

and the peak of C. intybus is at 1 660 cm-1 which from the protein amide I band

the intensity of peaks at 1 593

1 509

1 421

1 377

1 336 cm-1 of C. glandulosum is more different from C. intybus. In 1 200-700 cm-1

there have similar peaks at the 717

713 cm-1

in addition

the strongest peak of C. glandulosum is at 1 063

957 cm-1

more different from C. intybus. The distinction of 2D IR correlation spectra is more obvious. In 1 800-1 500 cm-1

the stronger automatic peaks of C. glandulosum present at 1 590

1 660 cm-1

respectively

but those of C. intybus have a very wide area auto peak in the 1 700-1 500 cm-1

respectively. In 1 120-880 cm-1

C. glandulosum has three automatic peaks while C. intybus has two automatic peaks. Conclusion: The infrared spectra of three identification method are simple

fast

can be used in the identification of C. glandulosum and C. intybus.