Objective: The content ofquercetin in Ilex kudingcha was determined by HPLC. Method: The separation was performed on a Kromasil C18 column (4.6 mm×250 mm
5 μm) with methanol-0.4% phosphoric acid (50: 50) as mobile phase
detected at 371 nm and 30℃. Result: Quercetinwas linear in the range of 5.98-47.84 g · mL-1 (r=0.999 7). Conclusion: The content ofquercetin in Ilex kudingcha could be determined by HPLC precisely.