Determination of Content of Trigonelline in Baogan Granulas
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Determination of Content of Trigonelline in Baogan Granulas
Chinese Journal of Experimental Traditional Medical FormulaeVol. 18, Issue 11, Pages: 129-131(2012)
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Published:2012
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LAN Wei, ZHAO Bao-sheng, JING Yu-xia, et al. Determination of Content of Trigonelline in Baogan Granulas[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 129-131.
DOI:
LAN Wei, ZHAO Bao-sheng, JING Yu-xia, et al. Determination of Content of Trigonelline in Baogan Granulas[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(11): 129-131.DOI:
Determination of Content of Trigonelline in Baogan Granulas
Objective: To establish a HPLC method for determination of trigonelline in Baogan granulas. Method: The content of trigonelline was determined by HPLC. The Agilent ZORBA×NH2 column (4.6 mm×250 mm
5 μm) was used and mobile phase was composed of water-methanol-acetonitrile(20 : 40 : 40). The flow rate of mobile phase was 1 mL·min-1 and detection wavelength was at 265 nm and the column temperature was maintained at 40 ℃. Result: The linear response range was from 5-25 μL(r=0.999 9). The recovery rate was 98.3%(n=9)
RSD was 1.63%. Conclusion: HPLC method is simple
accurate and exclusive
which can provide the scientific basis for quality control of the Baogan granulas.
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